基于ISSR分子标记的海南凤仙花种群遗传多样性-论文.pdf

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1、热带作物学报2014，35(6)：1041—1046ChineseJournalofTropicalCrops基于ISSR分子标记的海南凤仙花种群遗传多样性钟云芳一，胡翔宇，一，宋希强，-，周兆德1海南大学热带作物种质资源保护与开发利用教育部重点实验室．海南海口5702282海南大学园艺园林学院．海南海口5702283海南大学农学院．海南海口570228摘要运用ISSR分子标记技术．分析海南岛石灰岩地区海南凤仙花自然种群的遗传结构，比较3个海拔梯度对种群遗传结构的影响。结果显示．13个ISSR引物的扩增结果显示其多态性位点百分率为97．7。AMOVA分析表明：海南风仙花自然种群的变异主

2、要来自种群内(92％)，而种群间变异较少(8％)；中海拔种群的遗传多样性最为丰富：而高海拔种群的遗传多样性则较低；种群的遗传距离和地理距离表现出显著的高度相关性。说明海拔是影响种群基因流的重要因素．高海拔种群可以向中低海拔种群进行传播花粉和种子等基因流动．而中低海拔种群向高海拔种群进行类似的基因流动则较为困难．海南风仙花数量较少的主要原因可能来自于其生境的限制。因此在以后的保护工作中要特别注重其生境的保护，避免人为因素的破坏。关键词海南凤仙花；遗传标记；海拔梯度；濒危机制中图分类号9948．12．2文献标识码AISSRAnalysisonPopulationGeneticDiversi

3、tyofImpatienshainanensisBalsaminaceae，anEndemicSpeciestoHainanIslandZHONGYunfang，HUXiangyu，SONGXiqiang，ZHOUZhaode'1KeyLaboratoryofProtectionandDevelopmentalUtilizationofTropicalCropGermplasmResources(HainanUniversity)MinistryofEd~atioa,Haikou,Hmnan570228，Chitin2CollegeofHorticuhureand0nc印eArchit

4、ecture，H~nanUniversity,Haikou,Hmnan570228,China3CollegeofAgronomy,HainanUniversity,Haikou,Hainan570228,ChinaAbstractImpatienshainanensisisendemictoHainanIsland．Itsnumberofplantsisverylowandhabitatiseasilydamaged．nlisresearchusedtheISSRmolecularmarkertechnologytoanalysisthegeneticdiversityofImpat

5、ienshainanensispopulationandcomparedtheeffectsofthreeelevationgradientsonpopulationgeneticstructure．Theresultsindicatedthat：13ISSRprimersamplificationresultsshowedthatthepolymorphismlocipercentageiS97．7％：AMOVAanalysisindicatedthatthevariationofImpatienshainanensispopulationoccurredmainlywithinpo

6、pulations(92％)andlimitedlybetweenpopulations(8％)；Ahitudepopulationsinthemostabundantgeneticdiversity；Thehighaltitudepopulationgeneticdiversityislow；geneticdistanceandgeographicdistanceofpopulationshowedasignificantlyhighspecificity．AnMysisshowsthataltitudeistheimportantfactorsthataffectthepopula

7、tiongeneflow，highaltitudepopulationscanbespreadpollenandseedstothelowaltitudepopulations，thesimilaractivityfromlOWaltitudepopulationstohighaltitudepopulationsiSmoredificult．themainreasonforthesmallnumberofImpatienshainanensi