基于代谢组学和蛋白组学技术的熊胆蛋白大分子物质对丙型肝炎治疗作用的研究-论文.pdf

《基于代谢组学和蛋白组学技术的熊胆蛋白大分子物质对丙型肝炎治疗作用的研究-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、Availableonlineatwww．sciencedirect．corn∞pChinese帮ScienceDirectJournalofNaturalMedicinesChineseJournalofNaturalMedicines2013，l1(6)：0653—0665一doi：10．3724／SP．J．1009．2013．00653MetabolomicsandproteomicsapproachestocharacterizeandassessproteinsofbearbilepowderforhepatitisCvirusWANGXi—Jun，YANGu

2、ang—Li，ZHANGAi—Hua，SUNHui，PIAOCheng—Yu，LIWei—Yun，SUNChang，WUXiu·Hong，LIXing—Hua，CHENYunNationalTCMKeyLabofSerumPharmacochemistry,KeyLaboratoryofChinmedomicsandMetabolomics,HeilongjiangUniversityofChineseMedicine，andKeyPharmacometabolomicsPla和ofChineseMedicines，Harbin150040,ChinaAvai1able

3、online20Nov．2013【ABSTRACT】Metabolomicsrepresentsanemergingandpowerfuldisciplinethatprovidesanaccurateanddynamicpictureofthephenotypeofbio—systemsthroughthestudyofpotentialmetabolitesthatcouldbeusedastherapeutictargetsandtOrth0discoveryofnewdrugs．HepatitisCvirus(HCV)isaleadingcauseofliver

4、diseaseworldwide，andisamajorburdenonpublichealthItishypothesizedthatananima1modelofHCVinfe：ctionwouldproduceuniquepatternsofendogenousmetabolites．Herein．amethodfortheconstructionofeficientnetworksispresentedwithregardtotheproteinsofbearbilepowder(PBBP)thatprotectagainstHCVasacasestudy．Ul

5、tra—performanceliquidchromatography，coupledwithelectrosprayionization，quadrupole—time—of—flighthighdefinitionmassspectrometry(UPLC—HDMS)，coupledwithpatternrecognitionmethodsandcomputationalsystemsanalysiswereintegratedtoobtaincomprehensivemetabolomicprofilingandpathwaysofthelargebiologic

6、aldatasets．Amongtheregulatedpathways，38biomarkerswereidentifiedandtwouniquemetabolicpathwayswereindicatedtobedifferentiallyafectedinHCVanimals．TheresultsprovidedasystematicviewofthedevelopmentandprogressionofHCV_andalsocouldbeusedtoanalyzethetherapeuticeffectsofPBBP’awidelyusedanti—HCVme

7、dicine．TheresultsalsoshowedthatPBBPcouldprovidesatisfactoryeffectsonHCVinfectionthroughpartiallyregulatingtheperturbedpathway．Themostpromisinguseinthenearfuturewouldbetoclarifythepathwaysforthedrugsandobtainbiomarkersforthesepathwaystohelpguidetestablepredictions．providei