不同工艺提取麻疯树籽油及其杀灭血吸虫尾蚴效果.pdf

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1、中国血吸虫病防治杂志2014年第26卷第2期ChinJSchistoControl2014，Vo1．26，No．2·187·[文章编号]1005．6661(2014)02-01874)3·短篇论著·不同工艺提取麻疯树籽油及其杀灭血吸虫尾蚴效果易平．一，余启枝，屈文，夏莹，程文华，李爽，刘嵌崎，童瑶，唐利军[摘要]目的观察不同工艺提取的麻疯树籽油杀灭血吸虫尾蚴的效果，以筛选制备麻疯树籽油的最佳工艺及配方。方法采用6种不同工艺提取的麻疯树籽油，进行杀灭血吸虫尾蚴即时接触试验。采用麻疯树籽油及其与不同添加剂制成的制剂，进行昆明小鼠血吸虫尾蚴即时接触感染试验，观察其抗血吸虫感染的减虫率。结果95％

2、乙醇回流、料液比1：8、2h提取工艺出油率达30．7％。即时接触试验6种麻疯树籽油30min内将尾蚴全部杀灭。即时接触感染试验麻风树籽油原液减虫率达70．97％，添加苯甲酸苄酯减虫率为58．87％，单一添加月桂氮酮减虫率达77．42％，添加苯甲酸苄酯、邻苯二甲酸二丁酯、月桂氮酮减虫率达100％。结论95％乙醇回流、料液比1：8、2hE取工艺为麻疯树籽油最佳提取工艺。麻疯树籽油有较好的血吸虫尾蚴杀灭作用。[关键词]日本血吸虫；尾蚴；麻疯树[中图分类号]R383．24[文献标识码]BJatrophaseedoilsextractedbydiferentmethodsandtheirefecto

3、nkillingcercariaofSchistosomajaponicumYIPing’2YUQu-zhi，QuWen。，XIAYing。，CHENGWen—hua。，LIShuang，LIUQin—qi，TONGYao，TANGLi-jun。1HubeiProvincialCeuterforDiseaseControlandPrevention，Wuhan，430079，China；2HubeiProvincialKeyLaboratoryforAp—pliedToxicology，Wuhan，ChinaCorrespondingauthor[Abstract]ObjectiveToe

4、valuatetheeffectofJatrophaseedoilsextractedbydifferentmethodsonkillingcercariaofSchisto—somajaponicumSOastoscreentheoptimumprocessandformulations．MethodsThecercariadirectlycontactingtestswithJatro—phaseedoilsextractedby6differentextractionprocesseswerecarriedout，andthemouseimmediatecontactingcerca

5、riainfectiontrialswithdifferentformulationsofJatrophaseedoilandvariousadditiveswereperformed．ResultsWith95％ethano1．theratioofmaterialtOliquidbeing1：8，and2hextraction，theoilextractionratewas30．7％．Thecercariadirectlycontactingtestsshowedthat6kindsofJatrophaseedoilkilledallcercariawithin30min．Inthemo

6、useimmediatecontactingcercariainfectiontrims，thewormdeclinedrateofJatrophaseedoilliquidwas70．97％，andthewormdeclinedrateofthesampleaddedwithbenzylbenzoatewas58．87％，andthewormdeclinedrateofthesampleaddedwithlaurocapramwas77．42％．Theworindeclinedrateofthesam—plesaddedwithbenzylbenzoate，dibutylphthalat

7、eandlaurocapramwas100％．ConclusionTheprocesswith95％ethanol，theratioofmaterialtoliquidbeing1：g，and2hextractionistheoptimum，andtheJatrophaseedoilhasagoodkillingschistosomecercariaeffect．[Keywords]Schistosomajaponicu