内源CTK和IAA平衡对水稻分蘖芽休眠与萌发的影响.pdf

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1、作物学报ACTAAGRONOMICASINICA2014，40(9)：1619—1628http：／／zwxb．chinacrops．org／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajourna1．net．cnDoI：10．3724／SP．J．1006．2014．01619内源CTK和IAA平衡对水稻分蘖芽休眠与萌发的影响罗宝杰”许俊旭丁艳锋李刚华刘正辉王绍华南京农业大学农学院／农业部南方作物生理生态重点开放实验室，江苏南京210095摘要：研究水稻抽穗期分蘖休眠与萌发转换过程中CTK和IAA含量及CTK／IAA值的变化，探讨内源激素平衡

2、对水稻分蘖的调控作用。采用外施6-BA打破分蘖芽休眠和去穗后外施IAA抑制分蘖芽萌发两种处理，创建分蘖芽休眠与萌发转换的材料，测定伸长节和伸长节分蘖芽中CTK和IAA的含量。6-BA处理打破了分蘖芽的休眠，显著提高了倒二节和倒二芽中CTK含量，降低了倒二节中IAA含量，对倒二芽中IAA含量影响不大，显著提高了节、芽中CTK／IAA值。去穗后外施IAA逆转了去穗对节、芽CTK含量提高效果，CTK含量维持在较低值，显著提高了倒二节中IAA含量，对倒二芽中IAA含量无显著影响，显著降低了节、芽中CTK／IAA值。CTK和IAA共同调控分蘖发生，较高的CTK／IAA值利于分蘖芽萌发，较低的

3、CTK／IAA值使其休眠，节中CTK／IAA值在2．6~2．8，芽中CTK／IAA值在4．8~5．0，可作为分蘖芽休眠与萌发转换的临界指标。关键词：水稻；分蘖芽；休眠与萌发；激素平衡EffectsofEndogenousHormoneBalanceonDormancyandGerminationofTillerBudLUOBao．Jie，XUJun—Xu，DINGYan—Feng，LIGang—Hua，LIUZheng—Hui，andWANGShao．HuaCollegeofAgriculture，NanjingAgriculturalUniversity／KeyLaborator

4、yofCropPhysiology&EcologyinSouthernChina，MinistryofAgricultureNanjing210095，ChinaAbstract：TheobjectionofthisexperimentwastostudythechangesofcontentsofCTK．IAAandtheratioofCTK／IAAduringheadingstage，andtoinvestigatetheregulationofendogenoushormonesinricetiller．Exogenous6一BAandIAAwereusedtoregulat

5、ethedevelopmentoftillerbuds．establishthetransferbetweendormancyandgermination．andmeasurethecontentsofCTKandIAAinelongatednodesandtillerbudslocatedattheelongatednodes，Theexogenous6一BAincreasedtheCTKcontentsinnodesandtillerbuds．decreasedtheIAAcontentinnodeshadlittleefrectinthecontentofIAAintille

6、rbuds．significantlyin．creasedratiosofCTK／IAAinbothnodesandtillerbuds．andpromotedthetransferfromdormancytogermination．ExternalIAAafterremovingpanicledecreasedCTKcontentsinnodesandtillerbuds．whichweremaintainedatalowleve1，increasedtheIAAcontentinbuds．andsignificantlydecreasedtheratioOfCTK／IAAinn

7、odesandtillerbuds．causingthetransferfromgerminationtodormancy．TillerdevelopmentisdeterminedbythebalancebetweenCTKandIAA．higherCTK／IAAratioisbenefittothet啪sferfromdormancytogermination．whilelowerCTK／IAAratioinhibitsthetransfer．TheratiosO