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1、第35卷第11期东北林业大学学报Vol.35No.112007年11月JOURNALOFNORTHEASTFORESTRYUNIVERSITYNov.20071)3种枸杞耐盐碱性的比较程广有侯杰唐晓杰周喜云(北华大学,吉林,132013)(浑春林业局营林处)摘要利用组织培养技术,研究了枸杞(LyciumchinenseM.)、新疆枸杞(LyciumdasystemumP.)、宁夏枸杞(Ly-ciumbarbarumL.)的萌发与增殖状况。在培养基中添加NaCl或NaOH,不仅延迟了种子发芽的时间,也降低了枸杞种＊＊子的发芽率。随着盐或碱质量分数的升高,萌发率降低。3种枸杞在盐或碱条
2、件下发芽率差异极显著(76.01/＊＊85.69)。新疆枸杞种子在含盐培养基中具有较高的萌发率,宁夏枸杞和新疆枸杞在碱胁迫下具有较强的发芽率,枸杞种子萌发率在盐或碱中均较低。3种枸杞的增殖能力在含盐或碱培养基中都下降,且盐的质量分数或pH＊＊＊＊值越高,增殖率越低。在盐或碱培养基中,3种枸杞之间的增殖率差异极显著(100.93/125.8)。新疆枸杞的增殖能力较强,宁夏枸杞和枸杞较弱。新疆枸杞具有较好的耐盐碱能力。关键词枸杞;盐碱;组织培养;萌发;增殖分类号S722.36Salt-alkalineToleranceofThreeSpeciesofLyciumbyTissueCult
3、ure/ChengGuangyou,HouJie,TangXiaojie(For-estryCollegeofBeihuaUniversity,Jilin132013,P.R.China);ZhouXiyun(theManagementDepartmentofHunchunForestryBureau)//JournalofNortheastForestryUniversity.-2007,35(11).-47～49AnexperimentwasconductedtostudythegerminationandpropagationofLyciumchinenseMill,L.da
4、systemumPojark,L.barbarumL.inMSmediumsupplementedwithdifferentconcentrationsofNaClorNaOH.ResultsshowthatNaClorNaOHnotonlypostponesseedgermination,butalsoreducesgerminationrate.Thegerminationratedecreaseswithincreasingconcentrationofsaltoralkaliinmedium.Thereismarkeddifferencesingerminationrate
5、sbetweenthesethreespeciesinthepresenceofNaClorNaOHinthemedium.ThegerminationrateofL.dasystemuminthemediumwithNaClishigher,andL.barbarumandL.dasystemuminthemediumwithalkalialsohavehighergerminationrates,whileL.chinenseinthemediumwithNaClorNaOHhasthelowestgerminationrate.Multiplicationcapacities
6、ofthethreespeciesinthemediumwithNaClorNaOHdecrease,andthemultiplicationratedecreaseswithincreasingsaltconcentrationorpH.SignificantdifferencesinmultiplicationratewerefoundbetweenthesethreespeciesinthemediumwithNaCloralkali.ThemultiplicationcapacityofL.dasystemumishigherthanthatoftheothertwospe
7、cies.Therefore,L.dasystemumhashighersalt-alkalineresistance.KeywordsLyciumL.;Salineoralkali;Tissueculture;Germination;Multiplication2全世界约有5.7亿hm土地具有不同程度的盐渍化,约实践提供依据。占可耕地面积的10%;中国约有0.3亿多公顷盐荒地和0.1[1]1材料与方法亿多公顷盐渍化土壤,约占全国土地面积的1/4。为了充分利用盐碱地和
