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1、基础研究食品科学2006,Vol.27,No.08129从蛋黄中提取及纯化卵磷脂许晓慧1赵彬侠2赵立新1(1.山东淄博矿业集团技术中心山东淄博2551202.西北大学化工系陕西西安710069)摘要本文以鸡蛋的卵黄为原料采用含水乙醇与乙醚提取通过丙酮脱水去油得到粗品卵磷脂进一步以氯仿/甲醇为洗脱液硅胶柱层析法分离纯化自制的粗品卵磷脂得到高纯度的纯白色固体卵磷脂由薄层色谱定性检测仅含一个斑点用薄层扫描仪定量检测纯度大于97.4%关键词蛋黄卵磷脂提取纯化层析法ExtractionandPurificationofPhosphatidylcholinefromEggYolkXUXiao-
2、hui1ZHAOBin-xia2ZHAOLi-xin1(1.TheTechnologyCenterofZiboMiningGroupLtd.,Zibo255120,China2.DepartmentofChemicalEngineering,NorthwestUniversity,Xian710069,China)AbstractWitheggyolkasrawmaterial,extractedwithaqueousethanolandether,andremovingwaterandoilwithacetone,acrudephospholipidsmixturewasobta
3、ined.Thenthethin-layerchromatographywasusedwithcolumnonsilicicacidtopurifythephospholipidsmixture.Eluatedwiththesolventmixture(choroform/methanol),ultimatelyahighlypurewhitelecithinwasobtained.Analysisedbythinlayerchromatography,thereisonlyonespot.Withthin-layerchromatography-scanningmethodtoa
4、ssaythepurityitishigherthan97.4%.Keywordsyolkphosphatidylcholineextractionpurificationchromatographyonsilicicacid中图分类号Q545.1文献标识码A文章编号1002-6630(2006)08-0129-04收稿日期2005-03-31作者简介许晓慧(1973-)女硕士主要从事精细化工及分离提取的研究和开发工作HypothesisPhytopathology,1969,59(2):223-227.[12]BKHwang,WDIbenthal,RHeitefuss.Age,r
5、ateofgrowth,carbohy-[4]SKGayed,NRosa.Levelsofchlorogenicacidintobaccocultivars,drateandaminoacidcontentsofspringbarleyplantsinrelationtotheirhealthyandinfectedwiththielaviopsisbasicola[J].Phytopathology,resistancetopowderymildew[J].PhysiologicalPlantPathology,1983,1975,65(10):1049-1053.22:1-14
6、.[5]VJHiggins,DGSmith.Separationandidentificationoftwo[13]朱广廉,钟海文,张爱琴.植物生理学实验[M].北京大学出版社,238-pterocarpanoidphytoalexinsproducedbyredcloverleaves[J].239.Phytopathology,1972,62(2):235-238.[14]蒋传葵,金承德,吴仁龙,等.工具酶的活力测定[M].上海科学技术[6]ToruShimomura,TokuzoHirai.TheeffectofPyrimidinesand5-出版社,1982.phenyla
7、zopyrimidinesonthemultiplicationotobaccomosaicvirus[J].[15]MehtaA,etal.5thinternationalcongressofplantpathology,In:Ab-Phytopathology,1960,50(5):344-345.stractsofPapers,1988,55:11-116.[7]CHiruki,PShukla.Mycoplasmalikabodiesassociatedwith
