亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤的抑制作用及对DAPK的影响-论文.pdf

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1、2013庄临床耳鼻咽喉头颈外科杂志27卷9期JClin0t0rhino1aryngo1HeadNeckSurg(China)·479··实验研究·亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤的抑制作用及对DAPK的影响*黄雪琴王晓刚胡俊丽周慧。周克元张月飞[摘要]目的：研究亚砷酸(AszO。)联合顺铂(DDP)对人鼻咽癌CNE一2Z裸鼠移植瘤的生长抑制作用及对死亡相关蛋白激酶(DAPK)的影响。方法：建立人鼻咽癌裸鼠移植瘤模型，随机分为对照组、AsO。组、DDP组和As。0。加DDP组，比较各组的抑瘤作用；并对标本分别进行光镜、原位末端标记(TUNEI)检测，采用实时荧光定量P

2、CR法与免疫组织化学方法检测DAPK的表达。结果：AsO和As。O。加DDP均能显著抑制人鼻咽癌裸鼠移植瘤的生长，诱导肿瘤细胞凋亡，并能上调DAPK的表达。结论：AsO。可抑制人鼻咽癌CNE-2Z裸鼠移植瘤的生长，该作用与诱导人鼻咽癌CNE-2Z裸鼠移植瘤细胞凋亡、上调DAPK的表达相关。As()。与DDP联用可起协同作用。[关键词]亚砷酸；鼻咽癌；死亡相关蛋白激酶；凋亡；裸鼠[中图分类号]R739．6[文献标志码]A[文章编号]1001—1781(2013)09—0479—05InhibitoryeffectOfarsenictrioxidecombinedwith

3、cisplatinonhumannas0pharyngealcarcinomaxenograftandDAPKinnudemiceHUANGXueqin‘WNGXiaogangH【，，Junli。ZHOUHui。ZH0UKeyuanZHANGYuefei(GuangdongMedicalCollege，Zhanjiang，524023，China；。Departmentofotolaryngology，Affili—atedHospitalofGuangdongMedicalCollege)Correspondingauthor：ZHANGYuefei，E～mail：

4、hxqcong@163．cornAbstractObjeetive：TostudytheinhibitoryeffectofArsenicTrioxide(As2O3)combinedwithdiamminedi—chloroplatinum(DDP)onthegrowthofhumannas0pharyngea1carcinomacellstrainCNE-2Zxenograftinnudemice，andtOexplorethepossibleeffectmechanismsoftheantitumor．Method：Themodelsofhumanpoorlyd

5、iffer—entiatednasopharyngealcarcinomainnudemicewereestablishedandrandomlydividedintofourgroups。controlgroup，As2O3group，DDPgroupandAs2O3+DDPgroup．Theeffectofantitumoroneachgroupwasstudied．Thespecimenobtainedfromthemiceweredetectedbyopticalmicroscopeandtdt—mediateddutprockendlabeling(tune

6、1)method．ExpressionofDAPKwasdetectedbyrealtime—PCRandimmunohistochemistry．Result：As2()3groupandAs2O3+DDPgroupcouldobviouslyinhibitthegrowthoftumor，inducetheapoptosisofhumannaso—pharyngealcarcinomacellandup—regulatetheexpressionofRASSFIA．Conclusion：As2O3cangreatlyinhibitthegrowthofhumann

7、asopharyngealcarcinomacellstrainCNEr2Zxenograftinnudemice，whichwererelatedtOtheinducedapoptosisofhumannasopharyngea1carcinomacellandup—regulatedexpressionofDAPKCombinationofAs2O3withDDPseemtObemoreeffective．Keywordsarsenictrioxide；nasopharyngealcarcinoma；death—associatedprotein