傅里叶变换近红外光谱结合偏最小二乘法测定血清甘油三酯含量-论文.pdf

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1、第34卷光学学报光学前沿～信息光学2O14年9月ACTAOPTICASINICA傅里叶变换近红外光谱结合偏最小二乘法测定血清甘油三酯含量董海胜张丽芬钟悦黄贱英陈斌中国航天员科研训练中心航天医学基础与应用国家重点实验室，北京100094、z北京航天城门诊部，北京1f)()o94J摘要以日常体检用血清样品为实验材料，以临床传统甘油磷酸氧化酶一过氧化物酶一4一氨基安替比林一酚法测定得到的甘油三酯含量作为参比值，同时采集了所有血清样品近红外光谱，采集范围为12500~4000cm，结合偏最小二乘法建立了血清甘油三酯定量模型。实验结果表明，变量范围选取10796．2～8246．

2、6cm，采用多元散射校正对光谱进行预处理，所建立的血清甘油三酯偏最小二乘定量校正模型的相关系数尺为o．9454，交叉验证校正标准差(RMSECV)为0．146；检验集预测标准差(RMSEP)为0．151，相关系数R为0．9068。结果表明，应用近红外光谱技术结合偏最小二乘法建立血清甘油三酯定量模型，用于未知血清样品甘油三酯含量的非破坏性检测是可行的。关键词光谱学；近红外光谱；偏最小二乘；血清；甘油三酯中图分类号0433．4文献标识码Adoi：10．3788／AOS20l434．sl30001DeterminationofSerumTriglyeerideContent

3、byFourierNearInfraredSpectr0sc0pyandPartialLeastSquaresDongHaishengZhangLifenZhongYue。HuangJianyingChenBinStateKeyLaboratoryofSpuceMedicineFundamentalsandApplication，ChinaAstronautResearchandTrainingCenter，BeUing100094，ChinaBeijingAerospaceCityOut—PatientDepartment，Beijing100094，ChinaAb

4、stractTakingthewholebloodobtainedfromdailyclinicalinspectionasexperimentalmaterials，triglycerideconcentrationofserumisdeterminedbytraditionalclinicalmethod：glycerolphosphateoxidase—peroxidase-4-aminoantipyrine—pheno1method．Nearinfraredspectroscopyofallserumsamplesarecollectedatthesameti

5、me．withtherangebetween12500～4000cm．Aquantitativemodelofserumtriglycerideconcentrationbynearinfraredspectroscopyisestablishedbycombiningwithpartialleastsquares(PLS)method．Experimentalresultsshowthatthepropervariablerangeisl0796．2～8246．6cm．andwiththemultiplicativescattercorrectionasspectr

6、umpretreatmentmethod，thequantitativePLScalibrationmodelforserumtriglycerideisestablished．ThecorrelationcoefficientR一0．9454，cross．validationcalibrationstandarddeviation(RMSECV)is0．146forthecalibrationset：thepredictedstandarddeviation(RMSEP)iS0．151，andcorrelationcoefficientR一0．9068forthet

7、estset．Theresultsshowthat，bytheapplicationofnearinfraredspectroscopycombinedwithpartialleastsquaresmethod，theserumtriglyceridequantitativemodeliSsuccessfullyestablishedwithhighaccuracyandstability，whichcanbeusedfornon—destructivetestingoftriglyceridecontentintheunknownserumsamp