河南小麦新品种的春化基因型鉴定-论文.pdf

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1、植物遗传资源学报2014，15(5)：1040—1045JournalofPlantGeneticResourcesDOI：10．13430／j．cnki．jpgr．2014．05．018河南小麦新品种的春化基因型鉴定张江花，高曼霞，许海霞，陈锋，崔党群(河南农业大学农学院／河南省粮食作物协同创新中心．郑州450002)摘要：为了了解河南省最新培育小麦品种春化基因的等位变异状况，本研究利用分子标记技术对河南省新培育的50份冬小麦新品系(种)的春化基因Vrn—A1、‰一B1、VrnD1和Vrn—B3住点的等位变异组成

2、进行了鉴定和分析。结果表明，所有参试小麦品种的Vrn．B3位点基因型均表现为隐性．48份小麦品种的Vrn-A1和Vrn．B1位点基因为隐性．42份小麦品种的Vrn—DJ位点基因为隐性，说明隐性基因在河南小麦中占据主导地位。其中，豫农2019、豫农2020、豫农2071、国麦301、平安08—8、百农69、囤麦3698、08漯33共8个小麦品种的Vrn．D位点基因均为显性的Vrn—Dla类型。小麦品系豫农2o53和豫农3052的Vrn-A1和Vrn．B1位点的春化基因均表现为缺失，进一步研究表明，这2份小麦新品系仍能

3、正常开花，但开花期比对照周麦18分别晚1d和2d．因此Vrn—A1和Vrn—B1并非小麦开花的必需基因。本研究将为黄淮麦区广适、高产小麦新品种的选育和推广提供参考。关键词：普通小麦：春化基因；开花期；等位变异AllelicVariationoftheVernalizationGenesinHenanNewBreadWheatCultivarsZHANGJiang-hua，GAOMan—xia，XUHai—xia，CHENFeng，CUIDang—qun(AgronomyCollege~CollaborativeIn

4、novationCenterofGrainCrops，HenanAgriculturalUnive~ity，Zhengzhou450002)Abstract：Atoalof50breadwheatcuhivarsoradvancedlinesfromHenanprovincewereusedtoidentifyallel—iCvariationofvernalizationresponsegenesattheVrn．A1，Vrn—BJ，Vrn．DI。andVrn—B3loci．Theresultsshowedtha

5、tallwheatcuhivarssurveyedpossessedrecessiveallelesattheVrn．B3locus．and48cuhivatspossessedrecessiveal—lelesattheVrn．A1andVrn．BJloci．and42cuhivarspossessedrecessiveallelesattheVrn—D1locus．ItsuggesttedthatrecessivealleleswerethemostprevalentattheVrn．A1，Vrn8，凡．D1，

6、andn—B3lociinHenanbreadwheatcuhivarssurveyed．WheatcuhivarswithdominantvernalizationgenesattheVrn．D1locusincluded8cuhivarsofYunong2019，Yunong2020，Yunong2071，Guomai301，Pingan08-8，Bainong69，Tunmai3698，and08Luo33．TwonewlinesYunong3052andYunong2053，developedinHenan

7、AgnculturalUniversity，showedthedeletionsofbothVrn．AandVrn』genes．However．thesetwocuhivarsstillcouldnormallyflowerinfieldbuttheyfloweredtwo．dayslaterthanZhoumai18．ItsuggesttedthatVrn-A1andVrn—Blgeneswerenotnecessaryforbreadwheatflowering．Thisstudycouldprovideuse

8、fulinformationforscreeninghigh—yieldandwide—adaptabilitywheatcuhivarsinChinesewheatbreedingprogram．Keywords：breadwheat；vernalizationgene；floweringtime；allelievariation春化是小麦品种的重要生长发