氧化乐果和甲萘威亚致死剂量对黄褐天幕毛虫2种解毒酶活性的影响.pdf

《氧化乐果和甲萘威亚致死剂量对黄褐天幕毛虫2种解毒酶活性的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、第42卷第7期东北林业大学学报Vo1．42No．72014年7月JOURNALOFNORTHEASTFORESTRYUNIVERSITYJu1．2014氧化乐果和甲萘威亚致死剂量对黄褐天幕毛虫2种解毒酶活性的影响)付莉胡春祥(东北林业大学，哈尔滨，150040)摘要室内条件下采用叶片药膜法分别测定氧化乐果和甲萘威对黄褐天幕毛虫(Malacosom。neustri。test口．cea)3龄幼虫的毒力；采用分光光度法测定2种药剂亚致死剂量对黄褐天幕毛虫的羧酸酯酶(CarE)．谷胱甘肽s一转移酶(GSTs)

2、活性的影响。结果表明：氧化乐果对黄褐天幕毛虫的24、48h的Lc，分别为33．975、8．298mg·L～，甲萘威24、48h的LC。分别为23．314、1．863mg·L～。经氧化乐果和甲萘威亚致死剂量处理后，幼虫体内的CarE在6～48h内均被不同程度抑制，其中处理12h活性降到最低，分别为对照的0．51、0．36倍：氧化乐果亚致死剂量对幼虫体内的GSTs表现为抑制一激活一抑制作用，在处理后24h对该酶的激活作用最强，为同期对照的1．37倍。亚致死剂量甲萘威处理612h内幼虫体内GSTs被激活，随

3、后逐渐被抑制。关键词黄褐天幕毛虫：亚致死剂量；羧酸酯酶；谷胱甘肽s一转移酶分类号$763．3EfectofSublethalConcentrationofOmethoateandCarbarylontheActivitiesofTwoDetoxifyingEnzymesinMalacosomaneustriatestacea／FuLi，HuChunxiang(NortheastForestryUniversity，Harbin150040，P．R．China)／／JournalofNortheastF

4、orestryUniversity．一2014．42(7)．一126—128．132Weadoptedleaf-dipmethodtoevaluateomethoateandcarbarylagainstthethirdinstarofMalacosomaneustriatestaceaandusedsepectrophotometrytoassesstheeffectofsublethalconcentrationofthetwopesticidesonactivitiesofcarboxyles．

5、teraseandglutathioneS．transferase．For24hand48htreatment，theLC，nofomethoatewere33．975and8．298mK·L～．andtheLC，nofcarbarylwere23．314and1．863mg·L～，respectively．TheactivitiesofCarEinlavaewereinhibitedatdifferentlevelwithin6-48htreatmentofsublethalconcentratio

6、nofomethoateandcarbary．ComparedtoCK。theactivitiesofCarEweredecreasedby51％and36％，respectively．after12htreatment，whichweredecreasedtothelowestleve1．Bythetreatmentofthesublethalconcentrationofomethoat．theactivityofGSTswasinhibitedfirstly．thenincreasedandin

7、．hibitedfinally．After24htreatmentwithsublethalconcentrationofomethoate．theactivitywasincreasedby1．37timescomparedtothatofCK，whichwasathighestleve1．Within6—12hoftreatmentwithsublethalconcentrationofcarbarv1．theactivityofGSTsinlarvaewasincreasedfirstly，an

8、dthendecreasedgradually．KeywordsMalacosomaneustriatestacea；Sublethaldoses：Carboxylesterase：GlutathioneS—transferase黄褐天幕毛虫(Malacosomaneustriatestacea)属果、甲萘威的合理使用和黄褐天幕毛虫的综合防治鳞翅目枯叶蛾科，其分布范围广，食性杂，危害重，主提供理论依据。要以幼虫危害柞树、柳树、杨树、桦树等阔叶树_1]，1