螯合剂对ni污染土壤中紫花苜蓿生长和土壤酶活性的影响

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1、安徽农业大学学报,2017,44(4):684-689JournalofAnhuiAgriculturalUniversity[DOI]10.13610/j.cnki.1672-352x.20170811.024网络出版时间：2017/8/1112:05:57[URL]http://kns.cnki.net/kcms/detail/34.1162.S.20170811.1205.048.html螯合剂对Ni污染土壤中紫花苜蓿生长和土壤酶活性的影响＊谢芳芳，李信用，夏焕焕，丁艳萍，李玲(新疆大学化学化工学院，乌鲁木齐830046)摘要：通过盆栽紫花苜蓿实验，考察EDTA

2、和柠檬酸（CA）对供试土壤中Ni各个形态分布、紫花苜蓿生理-1指标和土壤酶活性变化的影响。结果表明，供试土壤中施加EDTA/CA浓度为5.0/2.5mmol·kg时，对土壤中Ni的活化效果较好，其水溶态含量是对照组的48.2倍，紫花苜蓿中丙二醛（MDA）含量提高了232.0%；施加EDTA/CA-1浓度为2.5/5.0mmol·kg时，紫花苜蓿转运系数最大，是对照组的194.3%；紫花苜蓿细胞通透性随螯合剂总添加量-1的增大而增大，施加EDTA/CA浓度为5.0/10.0mmol·kg时最大，比对照组增大206.3%；施加EDTA/CA对土壤过氧化氢酶均有不同程度的抑

3、制作用。土壤蔗糖酶酶的激活率与CA施加量显著相关（P＜0.01）。研究认为，将EDTA/CA适当配比后施加，既可以减少螯合剂的用量，又可以较好地提高紫花苜蓿富集土壤中重金属Ni的效果。关键词：重金属Ni；丙二醛；电导率；土壤酶活性；析因设计中图分类号：X53文献标识码：A文章编号：1672352X(2017)04068406InfluenceofchelatingagentsonalfalfagrowthandenzymeactivityinNipollutedsoilXIEFangfang,LIXinyong,XIAHuanhuan,DINGYanping,L

4、ILing(CollegeofChemistryandChemicalEngineering,XinjiangUniversity,Urumqi830046)Abstract:PotexperimentsofalfalfawereconductedtostudytheeffectsofEDTAandCAondistributionofNi,physiologicalindexesofalfalfaandchangesofsoilenzymeactivities.Theresultsshowedthatwhenthe-1EDTA/CAcontentwas5.0/2.5m

5、mol·kg,theNiinthesoilwasbetterhydratedwiththesolubilitywas48.2timeshigherthanthatofthecontrolgroup,andthecontentofmalondialdehyde(MDA)inalfalfaincreasedby232.0%-1comparedthecontrolgroup.WhentheconcentrationofEDTA/CAwas2.5/5.0mmol·kg,thetransfercoefficientofalfalfawasthehighest,whichwas1

6、94.3%ofthecontrolgroup.ThecellpermeabilityofalfalfaincreasedwiththeincreaseofthetotalamountofchelatingagentwiththehighestcellpermeabilityobservedinthegroupofEDTA/CA5.0/10.0(P<0.05),whichwas206.3%higherthanthatofthecontrolgroup.TheeffectofEDTA/CAonsoilcatalasewasinhibitoryindifferentexte

7、ntandtheinvertasewassignificantlycorrelatedwithCA(P<0.01).ItissuggestedthattheadditionofEDTA/CAinanappropriateproportioncanreducethedosageofchelatingagentandimprovetheenrichmentofheavymetalNiinalfalfa.Keywords:metalNi;MDA;elativeconductivity;soilenzymeactivity;fatorialdesign目前许