褐色橘蚜在健康与CTV植株上的EPG比较.pdf

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1、山地农业生物学报33(2)：036～039，2014JournalofMountainAgricuhureandBiology褐色橘蚜在健康与CTV植株上的EPG比较何应琴，陈文龙h，鲁卓越，周常勇，李中安，王雪峰，李太盛(1．贵州大学昆虫研究所，贵州山地农业病虫害重点实验室，贵州贵阳550025；2．中国农业科学院柑桔研究所，重庆北碚400712)摘要：利用刺探电位图谱(EPG)技术对褐色橘蚜Toxopteracitricida(Kirkaldy)在健康与感染CTV的植株上的取食行为进行了比较研究。结果显示，褐色橘蚜在二者上均产生8种取食波形，分别为非刺探波(np波)、路径波(A波、B波、C

2、波)、Pd波、韧皮部分泌唾液波(El波)、韧皮部被动吸食波(E2波)以及木质部主动吸食波(G波)。刺探过程中，二者非刺探总时间(np波)存在差异；褐色橘蚜在健康植株上的刺探次数最多且c波的总持续时间最长，与感病植株差异显著。感病植株上，E1波的次数和持续时间均显著多于健康植株。褐色橘蚜在感病植株上E2波的总持续时间为(241．33±24．12min)，显著长于在健康植株上(160．30±24．63min)的持续时间。由此可初步推断，感染CTV的植株在一定程度上会增加褐色橘蚜获毒与传毒的几率。关键词：褐色橘蚜；刺吸电位图谱(EPG)；取食行为；传毒；柑橘衰退病毒中图分类号：$433．3文献标识码

3、：A文章编号：1008—0457(2014)02—0036—04EPGanalysisoftheToxopteracitricidaonhealthyandCTV——infectedcitrusHEYing．qin，CHENWen1ong，LUZhuo—yue，ZHOUChang—yong，LIZhong—an，WANGXue-feng，LITai．shen(1．GuizhouKeyLaboratoryofPlantDiseaseandPestManagementforMountainAgriculture，Institu—teofEntomology，GuizhouUniversity，Gu

4、iyangGuizhou550025，China；2．CitrusResearchInstitute，ChineseA—cademyofAgriculturalSciences，BeibeiChongqing400712，China)Abstract：FeedingbehaviorsofToxopteracitricida(Kirkaldy)onhealthyandCTV—infectedcitruswereinves—tigatedbyelectricalpenetrationgraph(EPG)technique．TheresultsexhibitedthattherewereeightE

5、PGwaveformsofthecitricidafeedingonhealthyandinfectedplants：Non—penetrationwaveform(waveformnp)，pathwaywaveforms(waveformA，BandC)，waveformPd，salivasecretioninphloem(waveformE1)，phloemsapingestion(waveformE2)，andxylemsapingestion(waveformG)．Duringtheprocessofthepenetration，thedurationofthenon—penetrat

6、ionperiodwaveforms(waveformnp)weredifferentbetweenthehealthyandthediseasedplants．PenetrationtimesandthedurationofwaveformConthehealthyplantswerebothsignificantlylongerthanthatonthediseasedplants．Nevertheless，numbersandtotaldurationofwaveformE1onthediseasedplantsweresignificantlylongerthanthoseontheh

7、ealthyplants(P<0．05)．TotaldurationofE2ofcitricidaonthediseasedplants(241．33±24．12min)wasobviouslylongerthanitonthehealthy(160．30±24．63min)．Hence，itcouldbeinferredthattheprobabilityofviIMSacquisitionan