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时间:2017-12-08
《艾灸"足三里"穴对衰老大鼠心、脑组织中sod、mda、lf 的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、湖南中医药大学学报2013年11月第33卷第11期JournalofHunanUniv.ofCMNOV.2013Vo1.33No.11艾灸“足三里"穴对——L—▲老大鼠心、脑组织中SODMDA.LF的影响谭杏.杨茜芸,林亚平(1.湖南中医药大学研究生院,湖南长沙410208;2.湖南中医药大学针灸推拿学院,湖南长沙410208)[摘要]目的探讨艾灸足三里穴延缓衰老作用及其与抗氧化之间的关系。方法将36只健康雄性SD大鼠按完全随机平均分为3组:空白对照组、衰老模型组、艾灸足三里组,每组l2只。空白对照组大鼠按0.12
2、5g/(kg·d),从颈背部皮下注射0.9%的生理盐水,连续6周,每天1次;衰老模型组及艾灸足三里组则按0.125g/(kg·d),从颈背部皮下注射10%的D一半乳糖,连续6周,每天1次。艾灸足三里组造模后每穴每天5壮,每次25min左右,隔天1次,共治疗15次空白对照组及衰老模型组大鼠捆绑固定25rain.隔天1次,共15次。采用黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)活性,用硫代巴比妥酸法检测丙二醛(MDA)含量,荧光分光光度法测定脂褐素(LF)水平。结果学习记忆测试结果显示,艾灸足三里组与衰老模型组比较(P
3、<0.01),差异有统计学意义;与空白对照组比较,衰老模型组大鼠心、脑组织中SOD活性明显降低(尸4、issn.1674—070X.2013.11.024.086.04Efectsofmoxibustionat“Zusanli"oncontentofSOD,MDAandLFofcardioandcerebraltissuesinsenileratsTANXing,YANGQianyun2,LINYaping(1.GraduateSchoolofEducation,HunanUniversityofCM,Changsha,Hunan410208,China;2.CollegeofAcupunctureandMassa5、ge,HunanUniversityofCM,Changsha,Hunan410208,China)(AbstraeCObjectiveToinvestigatetheeffectsofdelayingsenilityofmoxibustionatZusanliacupointandtherelationshipbetweenitseffectsandanti-oxidation.Methods36healthymaleSDratswererandomlydividedintoblankcontrolgroup,a6、gingmodelgroup,moxibustionatZusan—ligroup,with12ratsineachgroup.Theratsinblankcontrolgroupweresubcutaneouslyinjectedwith0.125g/(kg·d)salineinthenapesandbacks1timeseveryday,continuouslyfor6W.Theothersweresubcutaneouslyinjectedwith0.125g/(kg·d110%D—galactoseinth7、enapesandbacks1timeseveryday,continuouslyfor6W.Aftermodeled,ratsinMoxibustionatZusanligroupweretreatedfor5strongeachacupointeveryday,eachstrongabout25min,1treatmenteveryoth—erday,atotalof15times.Theratsinblankcontrolgroupandagingmodelgroupareboundandfixed25min8、,1treatmenteveryotherday,atotalof15times.Superoxidedismutase(SOD)activity(收稿日期]2013—09—13[基金项目]湖南省中医药管理局课J~(201132)。[作者简介]谭杏(1986~),女,湖南湘潭人,在读硕士研究生,主要从事经络一脏腑相关规律与机制研究。[通讯作者]杨茜芸,女,医
4、issn.1674—070X.2013.11.024.086.04Efectsofmoxibustionat“Zusanli"oncontentofSOD,MDAandLFofcardioandcerebraltissuesinsenileratsTANXing,YANGQianyun2,LINYaping(1.GraduateSchoolofEducation,HunanUniversityofCM,Changsha,Hunan410208,China;2.CollegeofAcupunctureandMassa
5、ge,HunanUniversityofCM,Changsha,Hunan410208,China)(AbstraeCObjectiveToinvestigatetheeffectsofdelayingsenilityofmoxibustionatZusanliacupointandtherelationshipbetweenitseffectsandanti-oxidation.Methods36healthymaleSDratswererandomlydividedintoblankcontrolgroup,a
6、gingmodelgroup,moxibustionatZusan—ligroup,with12ratsineachgroup.Theratsinblankcontrolgroupweresubcutaneouslyinjectedwith0.125g/(kg·d)salineinthenapesandbacks1timeseveryday,continuouslyfor6W.Theothersweresubcutaneouslyinjectedwith0.125g/(kg·d110%D—galactoseinth
7、enapesandbacks1timeseveryday,continuouslyfor6W.Aftermodeled,ratsinMoxibustionatZusanligroupweretreatedfor5strongeachacupointeveryday,eachstrongabout25min,1treatmenteveryoth—erday,atotalof15times.Theratsinblankcontrolgroupandagingmodelgroupareboundandfixed25min
8、,1treatmenteveryotherday,atotalof15times.Superoxidedismutase(SOD)activity(收稿日期]2013—09—13[基金项目]湖南省中医药管理局课J~(201132)。[作者简介]谭杏(1986~),女,湖南湘潭人,在读硕士研究生,主要从事经络一脏腑相关规律与机制研究。[通讯作者]杨茜芸,女,医
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