A＋OSA污泥减量工艺的微生态特性.pdf

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1、第6卷第6期环境工程学报Vo1．6．No．62012年6月ChineseJournalofEnvironmentalEngineerinJun．2012A+OSA污泥减量工艺的微生态特性贾丽郭劲松高旭范莹翟小敏(重庆大学城市建设与环境工程学院，重庆400045)摘要采用16SrDNA序列与PCR．DGGE(polymerasechainreaction—denaturinggradientgelelectrophoresis)分析技术相结合的方法，研究了A+OSA(theanoxic+oxic—settling．anaerobic)

2、污泥减量工艺在不同工况下的减量效果及其微生态特性。结果显示，在自然条件下，A+OSA工艺可有效减少剩余污泥27％左右。分子生物研究表明，解耦联池的插入可以明显改变系统微生物的群落结构，且随着解耦联池水力停留时间的延长，系统中部分微生物被“淘洗”，微生物丰富度和多样性指数均有所降低。相似性分析表明，参照系统和A+OSA工艺分属于2个不同的集群，但在A+OSA工艺内部各反应池样品间具有较高的相似性，且各反应池在HRT为5．16h和7．14h时，表现为显著相似。通过上述研究可为该工艺优化及调控提供理论指导。关键词OSA工艺剩余污泥减量微生

3、物群落结构PCR—DGGE中图分类号X703文献标识码A文章编号1673—9108(2012)06—2049-06Microecologyofananoxic+oxic—settling-anaerobicsludgereductionprocessJiaLiGuoJingsongGaoXuFanYingZhaiXiaomin(FacuhyofUrbanConstructionandEnvironmentalEngineering，ChongqingUniversity，Chongqing400045，China)AbstractT

4、hemicrobialcommunitystructureandbiodiversityinananoxic+oxic。settling—anaerobic(A+OSA)processwerestudiedbypolymerasechainreaction(PCR)一denaturinggradientgelelectrophoresis(DGGE)basedon16SrDNAsequence．Theactivatedsludgesampleswerecollectedfromdifferentreactiontanksundero

5、perationconditions，andtheminimizationofexcesssludgeproductionoftheA+OSAprocesswasinvestigated．TheexperimentalresultsdemonstratedthattheA+OSAprocesscanreduceexcesssludgeby27％．TheDGGEprofilesshowedthatthemicrobialcommunitieswereafectedbytheinsertionoftheuncouplingtank．Mo

6、reover，therichnessindexandtheShannon’Sindexofdiversitydecreasedwithincreasingthehydraulicretch—tiontime(HRT)intheuncouplingtank．Thesimilarityanalysisrevealedthatthesamplesfromthereferencebe—longedtooneclusterandthesamplesfromtheA+OSAprocessbelongedtotheother．Additional

7、ly，thebacteri—alcommunitiesf0undinsamplesfromtheA+OSAoperatedunderdiferentconditionswerestatisticallysimilara-mongstthemselves，especiallyforthesamplesattheHRTof5．16hand7．14h．Thisstudywillprovidescientif-icguidanceforprocessoptimizationandapplication．Keywordsoxic-settli

8、ng—anaerobic(OSA)；sludgereduction；microbialcommunitystructure；PCR．DGGE污水生物处理过程中产生大量的剩余污泥，其应用中具有广阔的前景。处理处置所需的费用可占整个污水处