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《栽培大豆和野生大豆及其回交后代苗期耐盐性分析-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第31卷第4期大豆科学Vo1.31No.42012正8月S0YBEANSCIENCEAug.2012栽培大豆和野生大豆及其回交后代苗期耐盐性分析李发院,田芳,张晓可,於丙军(南京农业大学生命科学学院,江苏南京210095)摘要:以逐代人工耐盐性筛选的栽培和野生大豆杂交组合(Lee68XN23227)F代株系3060为父本,以耐盐栽培大豆Lee68品种为母本或轮回亲本,配置回交组合(Lee68X3060,编号c)得到的不同单株后代株系(BCF)为试验材料,采用苗期耐盐系数、相对生长速率、干物质积累量等指标,对它们的耐盐性进行了分析和评价,并采用相对电
2、解质渗漏率、硫代巴比妥酸反应物(TBARS)含量、叶片净光合速率(Ph)和PsⅡ最大光化学效率(Fv/Fm)对其中优良株系耐盐的生理机制进行了分析和探讨。结果表明:在盐胁迫下,该组合回交后代中C3、C10等株系的苗期耐盐性系数、相对生长速率和干物质积累量等指标均明显高于母本Lee68,接近或超过父本3060,其中C10株系尤为突出。与双亲相比,盐胁迫使CIO株系幼苗根和叶中相对电解质渗漏率和TBARS含量的上升幅度,及叶片尸n和Fv/Fm值的降低幅度明显较低,且盐胁迫下的相对电解质渗漏率和TBARS含量与其对照相比,差异均未达显著水平。可认为C10
3、株系是一种新的优良耐盐大豆种质材料。关键词:栽培大豆;野生大豆;回交后代;耐盐性中图分类号:$565.1文献标识码:A文章编号:1000-9841(2012)04-0593-05AnalysisofSeedlingsSaltToleranceofBackcrossHybridsofGlycinemaxandGlycinesojaLIFa—yuan,TIANFang,ZHANGXiao—ke,YUBing-jun(CollegeofLifeSciences,NanjingAgricuhuralUniversity,Nanjing210095,Chin
4、a)Abstract:Inthisstudy,thehybridstrain(F5)3060selectedforsalttolerancegenerationbygenerationfromthecrosscombina—tion(Glycinemax,Lee68cultivarXGlycines咖,N23227population)wasusedasthemaleparent,theLee68wasusedasthefemaleparentorrecurrentparent,thebackcrosscombination(Lee68×3060,
5、namedasC)wasconfigured,andseveralsinglehybridstrains(BClF2)wereobtained.Usingthesesoybeanmaterials,theirsalttolerancewasevaluatedwithparametersinclu·dingthesalttolerancecoefficient,relativegrowthrateanddrymatteraccumulation.Onthatbasis.thesalttolerancemechanismofonefinestrainw
6、asinvestigatedwithparameters.suchastherelativeelectrolyticleakage.thiobarbituricacidreactivesub—stances(TBARS)content,PnandthemaximumphotochemicaleficiencyofphotosystemII(Fv/Fm).Theresultsshowedthat,undersaltstress.thesalttolerancecoeficient.relativegrowthrateanddrymatteraccum
7、ulationinC3andC10hybridstrainswereobviouslyhigherthanthoseintheirfemaleparentLee68.andalmostbeequaltoormorethanthoseinthemaleparent3060strain,especiallytheC10straindisplayedthemost.Whencomparedwiththeirtwoparents,theincreaseoftherelativee.1ectrolyticleakageandTBARScontentinroo
8、tsandleaves.andthedecreaseofPnandFv/FminleavesofCIOseedlingsu
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