茉莉酸处理菜豆对美洲斑潜蝇抗性的影响-论文.pdf

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1、第40卷第4期植物保护学报Vo1．40No．42013年8月ACTAPHYT0PHYLACICASINICAAug．2013茉莉酸处理菜豆对美洲斑潜蝇抗性的影响田旭涛张箭李丹成卫宁(西北农林科技大学，农业部西北黄土高原作物有害生物综合治理重点实验室，陕西杨凌712100)摘要：为明确外源茉莉酸(JA)处理莱豆对美洲斑潜蝇抗性的诱导作用，采用生物测定和生化分析方法，研究了4种浓度JA喷施菜豆幼苗后，莱豆对美洲斑潜蝇的抗性及菜豆叶片过氧化物酶(POD)、多酚氧化酶(PPO)和脂氧合酶(LOX)活性的

2、变化。结果表明，JA处理可以提高菜豆对美洲斑潜蝇的抗性及菜豆叶片3种防御酶的活性，且其抗性和防御酶活性提高程度均与JA浓度有关。0．1、1mmoL／LJA可显著降低美洲斑潜蝇成虫对菜豆的取食和产卵选择性，使卵或幼虫发育历期延长、幼虫取食量降低、蛹重减轻，而0．001和0．01mmoL／LJA仅对幼虫发育历期有显著影响。O．1mmol／LJA处理后，菜豆叶片POD、PPO和LOX活性均显著升高，分别较对照增加33％、39％和8O％，而0．001mmol／LJA对菜豆叶片防御酶诱导作用不明显。关键词

3、：茉莉酸；菜豆；美洲斑潜蝇；诱导抗性；防御酶EfectsofexogenousjasmonicacidonresistanceofkidneybeantoLiriomyzasativaeTianXutaoZhangJianLiDanChengWeining(KeyLaboratoryofIntegratedPestManagementonCropsinNorthwesternLoessPlateau，MinistryofAgriculture，NorthwestA&FUniversity，Yan

4、gling712100，ShaanxiProvince，China)Abstract：Toclarifytheinductiveeffectofexogenousjasmonicacid(JA)ontheresistanceofkidneybeantoLiriomyzasativaeBlanchard，JAwassprayedonseedlingsofkidneybeanwithfourconcentra—tions，andinvestigatedtheresistanceofkidneybea

5、nto．sativaeaswellastheactivitiesofpolyphenoloxidase(PPO)，peroxidase(POD)andlipoxygenase(LOX)inkidneybeanleavesbymeansofbioassayandbiochemicalanalysis．TheresultsshowedthatthesprayedexogenousJAcouldincreasetheresistanceofkidneybeantoL．sativaeandtheacti

6、vitiesofthreedefensiveenzymesinkidneybeanleaves．TheeffectsonitsresistancetoL．sativaeandontheactivitiesofthreedefensiveenzymesweresignificantlyre—latedtoJAconcentration．Afterapplicationof0．1and1mmol／LJA，feedingandovipositionselectivity，feedingamountan

7、dpupalweightweresignificantlyreduced，whereasthedevelopmenttimeofeggsorlar—vaewassignificantlyprolonged．However，developmentaldurationoflarvaewasonlysignificantlyaffectedwhen0．01and0．001mmol／LJAwereapplied．Incomparisonwiththecontrol，theactivitiesofPPO，

8、PODandLOXwereincreasedby33％，39％and80％afterapplicationof0．1mmol／LJA，respectively．TheactivitiesofPPO，PODandLOXwerenotsignificantlyaffectedwhen0．001mmol／LJAwasapplied．Keywords：jasmonicacid；kidneybean；Liriomyzasativae；inducedresistance；defensiveenzyme基金项