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1、作物学报ACTAAGRONOMICASINICA2011,37(12):1−9http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2011.0000014个小麦品种(系)抗叶锈性分析**胡亚亚张娜李林懋杨文香刘大群河北农业大学植物病理学系/河北省农作物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心,河北保定071001摘要:选用16个小麦
2、叶锈菌菌系对14个小麦品种(系)进行抗叶锈性鉴定和苗期抗叶锈基因推导,初步分析这些品种(系)的抗性和携带的抗病基因;进一步利用21个与Lr基因紧密连锁或共分离的分子标记,对这14个品种(系)中可能含的抗叶锈基因进行鉴定。结果表明,s98351-2-2-2-1可能含Lr3a、Lr28和Lr50;9629-03A-4-1-1可能含Lr37;97167-1-2-1-1-2-1、919-20-2c2、9589、免中438、9916-8-6和9916-8-18含Lr26;96104-1-5-1c2可能含Lr28;00-55-3-
3、1-1含Lr1;1R13可能含Lr24、Lr37和Lr38;1R17可能含Lr24和Lr38;1R35含Lr10和Lr34,还可能含Lr3a和Lr50;9524-1-2-2-1含未知抗叶锈基因或本试验使用的已知抗病基因以外的抗叶锈基因。所有品种(系)均不含Lr9、Lr19、Lr20、Lr21、Lr29、Lr35、Lr42和Lr47基因。测试的14个品种(系)中有比较丰富的抗叶锈病基因,可为育种提供丰富的抗源。关键词:小麦叶锈;抗病基因;基因推导;分子标记辅助选择AnalysisofWheatLeafRustResist
4、anceGenesin14WheatCultivarsorLines**HUYa-Ya,ZHANGNa,LILin-Mao,YANGWen-Xiang,andLIUDa-QunDepartmentofPlantPathology,AgriculturalUniversityofHebei/BiologicalControlCenterofPlantDiseasesandPlantPestsofHebeiProvince/NationalEngineeringResearchCenterforAgricultureinN
5、orthernMountainousAreas,Baoding071001,ChinaAbstract:Theobjectiveofthisstudywastodetectresistancegenestoleafrustin14wheatcultivarsorlines.Theresistanceofthe14wheatcultivarsorlineswasinvestigatedatseedlingandadultstages,andtheresistancegenesatseedlingstagewerepost
6、ulatedbyinoculating16racesofPucciniatriticina.Furthervalidationwasconductedbyusing21molecularmarkerscosegregatedwithorcloselylinkedtotheknownLrgenes.Accordingtotheresultsofbothphenotypicidentificationandmolecularmarkerdata,theinformationoftheresistancegenesinthe
7、14cultivarsorlineswasobtained.GenesLr3a,Lr28andLr50werepostulatedins98351-2-2-2-1.GeneLr37waspossiblycarriedby9629-03A-4-1-1.Lr26waspresentin97167-1-2-1-1-2-1,919-20-2c2,9589,Mianzhong438,9916-8-6,and9916-8-18.Lr28waspossiblypresentin96104-1-5-1c2.Lr1wascontaine
8、din00-55-3-1-1.Lr24,Lr37,andLr38werepossiblyin1R13.Lr24andLr38werepossiblycarriedby1R17.GeneLr10andLr34weredetectedin1R35,andLr3aandLr50werealsopostulatedin1R35.Besid