不同绿僵菌菌株对筛胸梳爪叩甲幼虫的毒力测定

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1、林业科学研究2014，27(1)：066～070ForestResearch文章编号：1001—1498(2014)01-0066-05不同绿僵茵菌株对筛胸梳爪叩甲幼虫的毒力测定张亚波，叶碧欢，刘振勇，张炜，舒金平(1．中国林业科学研究院亚热带林业研究所，浙江富阳311400；2．浙江省德清县森林病虫害防治检疫站浙江德清313299；3．陕西省铜川市森林病虫害防治检疫站，陕西铜川727007)摘要：竹林金针虫是南方竹林笋期最为重要的害虫之一，筛胸梳爪叩甲幼虫是竹林金针虫的优势种群，应用绿僵菌防治具有重要意义。测定了3株绿僵菌对筛胸梳爪叩甲幼虫的毒

2、力，并比较了毒土法和毒饵法两种施菌方式对绿僵菌致病力的影响。结果表明，平沙绿僵菌WP08菌株和金龟子绿僵菌30104菌株对筛胸梳爪叩甲幼虫具有明显的致病效果，在30d的试验期内，校正累计死亡率分别为90．0％和66．3％，半数致死时间()分别为15．8d和25．8d；毒土法的校正累计死亡率略高于毒饵法，分别为60．1％和52．O％，L分别为22．6d和28．0d。金龟子绿僵菌LRC112菌株对金针虫无致病力。关键词：绿僵菌；金针虫；毒力测定；生物防治中图分类号：$763文献标识码：AVirulenceBioassayofDiferentMetar

3、hiziumStrainsAgainstMelanotuscribricoLarvaeZHANGYa-bo。YEBi—huan，LIUZhen．yong，ZHANGWei，SHUfin-ping。，(1．ResearchInstituteofSubtropicalForestry，ChineseAcademyofForestry，Fuyang311400，Zhejiang，China；2．ForestPestManagementandQuarantineStationofDeqingCounty，ZhejiangProvinc，Deqing313

4、299，Zhejiang，China；3．ForestPestManagementandQuarantineStationofTongchuanCity，ShaanxiProvince，Tongchuan727007，Shaanxi，China)Abstract：ThebamboowirewormMelanotuscribricollisiSadevastatingpestofundergroundbambooshootsinsouth．ernChina．Toexploreanalternativecontrolstrategywithfunga

5、lbiocontrolagents，theauthorsbioassayedthevim—lenceofthreeMetarhiziumstrainsagainstMelanotuscribricollislarvae(120—150mgpercapita)insmallcupsfilledwith50gsoilcontaining5×10。conidiapergramofsoil．TheapplicationofthestainsMetarhiziumpingshaenseWPo8andMetarhiziumanisopliae30104res

6、ultedinthecorrectedmortalitiesof9O．0％and66．3％duringa30一dayperiodundercontrolledconditions，respectively，whereasMetarh&iumanisopliaeLRC112showednolethaleffectonthelarvae．Time．mortalityanalysisledtothemedianlethaltime(Ln)estimatesof15．8and25．8daysforthetwovirulentstrains．Thehigh

7、—virulencestrainMetarhiziumpingshaenseW1：'08wasfurtherassayedforitsle—thaleffectonthelarvaeincontactofconidiamixedwithcornbaitandsoilrespectively．Asaresult．thesoilmixturecausedhighermortality(60．1％)andshorterL0(22．6days)thanthebaitmixture(52．0％；28．0days)．Keywords：Metarhizium；

8、Melanotuscribricollis；virulencetest；biologicalcontrol金针虫是叩甲(鞘翅目Coleo