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1、昆虫学报ActaEntomologicaSinica,June2005,48(3):380-385ISSN045426296TheavailabilityofmitochondrialDNAcytochromeoxidaseⅠgeneforthedistinctionofforensicallyimportantfliesinChina11123CAIJi2Feng,LIUMin,YINGBin2Wu,DENGReng2Li,DONGJian2Guo,111113ZHANGLin,TAOTao,PANHong2Fu,YANHong2Tao,LIAOZ
2、hi2Gang(11WestChinaSchoolofPreclinicalandForensicMedicine,SichuanUniversity,Chengdu610041,China;21WestChinaSchoolofClinicalMedicine,SichuanUniversity,Chengdu610041,China;31SchoolofPreclinicalMedicine,ShanghaiSecondMedicalUniversity,Shanghai200025,China)Abstract:Sarcosaphagousfl
3、iesarefrequentlyfoundondeadbodiesshortlyafterdeath.Specieswithinthisfamilydifferintheirdevelopmentaltimes,soanaccurateidentificationofeveryspeciesisnecessaryforthecorrectestimationofthepostmorteminterval(PMI).Identificationofspecimensistraditionallyperformedusingtheirmorphologi
4、calfeatures.Themorphologicalsimilarityofsarcosaphagousfliesespeciallytheireggs,larvaandpupae,posesachallengeforforensicentomologists.Thereforeamolecularmethodwasestablishedforspeciesidentification.Inthisstudy,A278basepairregionofmitochondrialDNA(mtDNA)codingforcytochromeoxidase
5、subunitone(COⅠ)wasinvestigatedforidentificationofthefollowingforensicallyimportantspeciesofsarcosaphagousfliesfromwesternChina,includingOphyracapensis(Wiedemann),Chrysomyamegacephala(Fabricius),Luciliasericata(Meigen),Luciliacuprina(Wiedemann),andBoettcheriscaperegrina(Robineau
6、2Desvoidy).TheresultsindicatedthattheCOⅠregionsequencedallowedidentificationofmajorspecies,providingseparationofcongenericspecieswithhighsupport.Incontrast,thedatacouldnotdistinguishtaxafromthesamespeciesgroup,i.e.theL.sericataandL.cuprinagroupsbecauseoflowsequencedivergence.Ke
7、ywords:Sarcosaphagousflies;speciesidentification;mitochondrialDNA;cytochromeoxidaseⅠAttheendofthe19thcentury,theFrenchfirsttothirdinstarlarvaeofsarcosaphagousfliescouldentomologistMegninandBeneckeusedsarcosaphagousnotbeidentifiedbymorphologyicalmethod(Wallmanfliesthathaddevelop
8、edondeadhorseslyinginafieldandDonnellan,2001).Underthe
