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时间:2017-12-07
《不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、第49卷第12期林业科学Vo1.49.No.122013年12月SCIENTIASILVAESINICAEDec.,2013doi:10.11707/j.1001—7488.20131215不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应术林英华汪来发卢萍谭飞。徐演鹏孙楠(1.中国林业科学研究院湿地研究所北京100091;2.中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京100091;3.东北林业大学野生动物资源学院哈尔滨150040;4.黑龙江省林业科学院哈尔滨150081)摘要:应用稀释平板法和16SRNA序列分析法对不同密度杂种落叶松长白落叶松
2、又日本落叶松人工林2种孔径凋落袋,即上层网孔2mm、下层网孔1mm的异孔分解袋和网孔均为1/300mm的同孔分解袋中凋落物分解过程中土壤细菌群落及多样性进行研究。结果表明:土壤细菌数量因凋落物分解而发生明显变化(F=10.97,P3、多样性与复杂性影响不一致。典型相关分析表明:土壤细菌群落生存的环境条件较为相似,土壤K对土壤细菌分布影响最大,土壤C/N对土壤细菌分布影响最小。关键词:落叶松;细菌群落多样性;凋落物分解袋;动态变化;土壤性质;典型相关分析中图分类号:$718.8文献标识码:A文章编号:1001—7488(2013)12—0101—07ResponseofSoilBacteriaCommunitytoLeafLitterDecompositionofLarch(Larixolg。ensis×Larixkaempferi)PlantationwithDiferentForestDensitiesLinYing4、huaWangLaifaLuPing·TanFei,XuYanpeng·。SunNan(1.InstituteofWetlandResearch,CAFBeijing100091;2.KeyLaboratoryofForestProtectionofStateForestryAdministrationResearchInstituteofForestEcology,EnvironmentandProtection,CAFBeijing100091;3.CollegeofWildlifeResource,NortheastUniversityofForestryHarbin150040;45、.HeilongjiangAcademyofForestryHarbin150081)Abstract:SoilbacteriacommunityandtheirdiversitywerestudiedduringtheleaflitterdecompositionofLarixolgensis×Larixkaempferiplantationwithdifferentdensitiesbytwomeshsizeoflitterbags,thatis,toplayerof2mmmeshandbottomlayerof1mmmesh(mixedmeshlitterbag),aswellas16、/300mmmeshsize(samemeshlitterbag)oflitterbags.ThebacteriawereinvestigatedwiththeplatecultivationmethodandPCR一16SRNAsequenceanalysis.Theresultshowedthatthenumberofsoilbacteriawaschangedsignificantly(F:10.97,P<0.01)duringleaflitterdecomposition.Thebacteriaparticipatingincellulose,hemicellulosedecomp7、osition,suchasBacillus,Pseudomonas,Staphylococcus,wasthemostdominant.Thevarietyofbacteriacommunitywasnotconsistentbetweenthetwotypesoflitterbags.Thenumberofbacteriainthesamemeshlitterbagincreasedwiththeplantation
3、多样性与复杂性影响不一致。典型相关分析表明:土壤细菌群落生存的环境条件较为相似,土壤K对土壤细菌分布影响最大,土壤C/N对土壤细菌分布影响最小。关键词:落叶松;细菌群落多样性;凋落物分解袋;动态变化;土壤性质;典型相关分析中图分类号:$718.8文献标识码:A文章编号:1001—7488(2013)12—0101—07ResponseofSoilBacteriaCommunitytoLeafLitterDecompositionofLarch(Larixolg。ensis×Larixkaempferi)PlantationwithDiferentForestDensitiesLinYing
4、huaWangLaifaLuPing·TanFei,XuYanpeng·。SunNan(1.InstituteofWetlandResearch,CAFBeijing100091;2.KeyLaboratoryofForestProtectionofStateForestryAdministrationResearchInstituteofForestEcology,EnvironmentandProtection,CAFBeijing100091;3.CollegeofWildlifeResource,NortheastUniversityofForestryHarbin150040;4
5、.HeilongjiangAcademyofForestryHarbin150081)Abstract:SoilbacteriacommunityandtheirdiversitywerestudiedduringtheleaflitterdecompositionofLarixolgensis×Larixkaempferiplantationwithdifferentdensitiesbytwomeshsizeoflitterbags,thatis,toplayerof2mmmeshandbottomlayerof1mmmesh(mixedmeshlitterbag),aswellas1
6、/300mmmeshsize(samemeshlitterbag)oflitterbags.ThebacteriawereinvestigatedwiththeplatecultivationmethodandPCR一16SRNAsequenceanalysis.Theresultshowedthatthenumberofsoilbacteriawaschangedsignificantly(F:10.97,P<0.01)duringleaflitterdecomposition.Thebacteriaparticipatingincellulose,hemicellulosedecomp
7、osition,suchasBacillus,Pseudomonas,Staphylococcus,wasthemostdominant.Thevarietyofbacteriacommunitywasnotconsistentbetweenthetwotypesoflitterbags.Thenumberofbacteriainthesamemeshlitterbagincreasedwiththeplantation
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