agei限制性内切酶使用说明书

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1、Home›Products›AgeIAgeIThisenzymehastransitionedtoanimprovednewbuffersystem.VisitNEBCutSmart.comforfurtherdetails.ThenewandcurrentDoubleDigestFinderandcurrentActivity/PerformanceChartfortheCutSmartbuffersystemareavailable.ThepreviousversionoftheDoubleDigestFinder,aswellasthepreviousVers

2、ionofActivity/PerformanceChartthatusetheformerbuffersystem,arestillavailableforyourconvenience.AgeIhasHighFidelity(HF)andRE-MixMasterMixversionsavailable.IsoschizomersCatalog#SizeConcentrationPriceQtyR0552S300units5,000units/ml$67.001R0552L1,500units5,000units/ml$268.001Categories:Rest

3、rictionEndonucleases:AApplications:RestrictionEnzymeDigestionProductFAQsProtocolsOtherTools&RelatedInformationResourcesProductsDescriptionPropertiesandUsageQualityControlDescriptionAgeIhasaHighFidelityversionAgeI-HF™(NEB#R3552).HighFidelity(HF™)RestrictionEnzymeshave100%activityinCutSm

4、art™Buffer;single-buffersimplicitymeansmorestraightforwardandstreamlinedsampleprocessing.HFenzymesalsoexhibitdramaticallyreducedstaractivity.HFenzymesareallTime-SaverqualifiedandcanthereforecutsubstrateDNAin5-15minuteswiththeflexibilitytodigestovernightwithoutdegradationtoDNA.Engineere

5、dwithperformanceinmind,HFrestrictionenzymesarefullyactiveunderabroaderrangeofconditions,minimizingoff-targetproducts,whileofferingflexibilityinexperimentaldesign.ProductSourceAnE.colistrainthatcarriestheAgeIgenefromRuegeriagelatinovora(ATCC25655).ReagentsSuppliedThefollowingreagentsare

6、suppliedwiththisproduct:NEBuffer1.110XPropertiesandUsageUnitDefinitionOneunitisdefinedastheamountofenzymerequiredtodigest1µgofλDNAin1hourat37°Cinatotalreactionof50µl.ReactionConditions1XNEBuffer1.1Incubateat37°C1XNEBuffer1.1:10mMBis-Tris-Propane-HCl10mMMgCl2100μg/mlBSApH7@25°CActivityi

7、nNEBuffersNEBuffer1.1:100%NEBuffer2.1:75%NEBuffer3.1:25%CutSmart™Buffer:75%DiluentCompatibilityDiluentCStorageTemperature-20°CStorageConditions10mMTris-HCl250mMNaCl1mMDTT0.1mMEDTA200μg/mlBSA50%Glycerol0.15%Triton®X-100pH7.4@25°CHeatInactivation65°Cfor20minMethylationSensitivitydammet