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1、2017,36(3):443-448农业环境科学学报2017年3月JournalofAgro-EnvironmentScience王亚丽,仪慧兰,韩彦莎.SO2对谷子幼苗根系镉胁迫的缓解作用[J].农业环境科学学报,2017,36(3):443-448.WANGYa-li,YIHui-lan,HANYan-sha.Sulfurdioxidealleviatescadmiumtoxicityintherootsoffoxtailmilletseedlings[J].JournalofAgro-EnvironmentScience,2017,36(3):443-448.SO2对谷子幼
2、苗根系镉胁迫的缓解作用*王亚丽,仪慧兰,韩彦莎(山西大学生命科学学院,太原030006)摘要:以谷子幼苗为材料,采用SO2---1/mmol·L-12衍生物(SO3∶HSO3,3∶1,mmol·L)预处理方式,研究外源SO2对镉(Cd)致根系毒性的影响。研究发现:250、500μmol·L-1Cd胁迫下,谷子幼苗根生长受到明显抑制,根组织中活性氧(ROS)大量产生,膜脂过氧化增加;与Cd单独处理组相比,用500μmol·L-1SOCd对根系生长的抑制作用减弱,根组织中ROS水平降低,膜脂氧2衍生物预处理后,化损伤减轻,谷胱甘肽(GSH)含量提高,过氧化物酶(POD)、谷胱甘肽过氧
3、化物酶(GPX)和谷胱甘肽硫转移酶(GST)等酶活性明显增加。结果表明:一定浓度的SO2衍生物能够通过上调抗氧化酶系统POD和GPX的活性来有效缓解Cd胁迫造成的谷子根系氧化损伤,并很可能通过维持较高的GSH水平和提高GST活性来增强谷子根系的Cd解毒能力。关键词:谷子;二氧化硫;镉;根系;抗氧化系统中图分类号:X503.231文献标志码:A文章编号:1672-2043(2017)03-0443-06doi:10.11654/jaes.2016-1338Sulfurdioxidealleviatescadmiumtoxicityintherootsoffoxtailmillets
4、eedlingsWANGYa-li,YIHui-lan*,HANYan-sha(SchoolofLifeScience,ShanxiUniversity,Taiyuan030006,China)Abstract:Sulfurisanessentialelementforplantgrowthandresponsestoenvironmentalstress.WeshowthatexogenousSOderivates(SO2-23∶HSO--1/mmol·L-1)alleviatedcadmium(Cd)toxicityinfoxtailmillet(Setariaitalica
5、L.)seedlings,mainlyfocusingonseedling3,3∶1,mmol·Lroots.Theresultsshowthat250μmol·L-1and500μmol·L-1Cdmarkedlyinhibitedrootgrowth,increasedtheproductionofreactiveoxygenspecies(ROS),andcausedhigherlevelsoflipidperoxidationproductmalondialdehyde(MDA)inrootsoffoxtailmilletseedling.ComparedtoCdtrea
6、tmentalone,pretreatmentwith500μmol·L-1SOderivatesweakenedtheinhibitoryeffectsofCdonrootlengthanddryweight,2anddecreasedtheROSandMDAlevelsinmilletrootsunderCdstress.Moreover,theactivitiesofperoxidase(POD),glutathioneperoxidase(GPX)andglutathioneS-transferase(GST)aswellasglutathione(GSH)content
7、sinSO2pretreatmentgroupswerealsosignificantlyhigherthanthoseinCdtreatmentalone.TheseresultsindicatethatactivitiesofPOD,GPXandGSTenzymes,andincreaseofGSHcontentinducedbySO2pretreatmentplayimportantrolesinplantadaptationtoCdstress.Ourresultssug