氧化石墨烯荧光淬灭.ppt

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1、GrapheneOxide−PeptideNanocomplexasaVersatileFluorescenceProbeofProteinKinaseActivityBasedonPhosphorylationProtectionagainstCarboxypeptidaseDigestion报告人：蔡向东1.ABSTRACTTraditionalmethodsofassessingkinaseacitivitiesGraphene&GrapheneOxide2.EXPERIMENTALSECTI

2、ON&RESULTSANDDISCUSSIONGraphiteoxide，staurosporine，forskolin，CKII，IBMX，PKA，CPY，H-89，BSA(NIUXUEQING),Tris,glycerol,DTT,EDTA,andTritonX-100，FITC-pep,FITC-Ppep,FITC-kemptideSynergyMxmultimodemicroplatereaderexcitationwavelengthof480nm,fluorescenceemissionw

3、asscannedfrom500to700nmFluorescenceQuenchingofFITCMoleculesandFITC-peptidebyGrapheneOxideAFMtopographicimages(top)andheightprofiles(bottom)ofGO(A)beforeand(B)afterincubationwiththe10μMpeptidesubstrateofCKII.HydrolyzationofPeptidebyCPY.Fluorescencerespo

4、nsesat520nmemissionof10μMFITC-labeledpeptide(S-pep)digestedbydifferentconcentrationsofCPYfor30minandthenmixedwithGO.DetectionofProteinKinaseActivityandInhibition.MCF-7CellCultureandLysatePreparationMCF-7breastcancercells+amphotericinB+penicillin+FskandI

5、BMX(DMSO)improvedBradfordproteinassaydyereagentkit+BSA3Q