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1、聚己内酯(PCL)论文：基于Web日志挖掘的高校图书馆门户网站建设研究【中文摘要】聚己内酯(PCL)是由ε-己内酯开环聚合制得的热塑性聚酯,由于PCL的熔点较低,热稳定性和水解稳定性优良,与多种聚合物的相容性很好等诸多优点,与其他脂肪族聚酯相比有着广阔的应用前景。但是,目前对于PCL生物降解的研究还很少,本文的重点是从环境中筛选出一株可生物降解PCL的细菌菌株,将其编号为DS0801,通过菌落形态、生理生化指标和16SrDNA技术对该菌株进行鉴定,并且使用蛋白分离纯化手段对DS0801菌株的PCL胞外解聚酶进行了分离纯化,对酶学性质进
2、行了表征。主要实验结果如下:1、PCL降解菌株的筛选鉴定。从环境污水中筛选出具有PCL降解能力的菌株,对菌种进行初步鉴定。通过菌落形态、生理生化指标和16SrDNA技术确定该菌为铜绿假单胞菌(Pseudomonasaeruginosa)。2.对PCL解聚酶进行分离纯化,并对其解聚酶粗酶的性质进行了探讨。实验证明,DS0801菌株的最佳发酵时间为36h,粗酶最适反应温度为40℃,最适反应pH值为8.0。3.用电子显微镜观察PCL膜的降解,在3d之内,PCL膜表面变化不大;在降解5d后,PCL膜表面开始发生断裂,表面发生严重侵蚀;降解7d
3、时,PCL膜失重率接近于100%,膜样品断裂成碎片,基本上完全降解。4.对粗酶的降解产物进行质谱分析,PCL解聚酶粗酶的降解产物中有单体ε-己内酯生成,失活的粗酶与PCL混合后没有单体ε-己内酯的出现,证明PCL解聚酶粗酶能够降解PCL,并且将其降解成ε-己内酯单体。【英文摘要】Polycaprolactone(PCL)isakindofthermoplasticpolyesterwhichispolymerizedbyε-caprolactone.PCLhasmanyexcellentcharacteristics,suchasth
4、elowmeltingpoint,thermalstabilityandhydrolysisstability,thecompatibilitywithvariouspolymersisverygood.Comparedwithotheraliphaticpolyesters,PCLhasbroadapplicationprospect.However,therearefewresearchesaboutthebiodegradationofPCL,thispaperfocusesonisolateabacterialstrainto
5、biodegradePCLfromtheenvironment,numbereditDS0801.PurifyandidentifythisstrainDS0801throughthecolonymorphology,physiologicalandbiochemicaltestesand16SrDNAtechnology.Inaddition,isolateandpurifyextracellularPCLdepolymerasesfromthestrainDS0801withthemeansofproteinpurificatio
6、n,studythefundamentalcharacteristicsofthePCLdepolymerases.Themainresultsobtainedfromthisworkareasfollows:1.TheisolationandidentificationofPCLdegradationbacterium.ThePCLdegradationstrainwasisolatedfromtheenvironmentwastes.Identifythestrainpreliminary.Throughthecolonymorp
7、hology,physiologicalandbiochemicaltestesand16SrDNAtechnology,wecandeterminethatthebacteriabelongsforPseudomonasaeruginosa.2.ThedepolymeraseofPCLwerepurified,andthepropertiesofitscrudeenzymewerediscussed.Theresultsoftheexperimentsshowthat,theoptimumfermentationtimeofthes
8、trainDS0801is36hours,theoptimumtemperatureofcrudeenzymeis40℃,andtheoptimumpHis8.0.3.ThePCLfilmisscannedbyelect
