蛋白质芯片技术介绍.ppt

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1、——Thefutureisinyourhands!TheproteinchipNowadays,thepoteomics(蛋白质的)researchisoneofageneraldirectionofthedevelopmentoflifescienceaftergenomics（基因组学的）research.Proteinstructureandfunctionhavethedirectimpactonthechangeoflifeactivity.Andthelevelofgenetranscript

2、ion(转录)canonlyreflectsthechangeofgeneexpressionproductsinacertainextent.However,trulyfunctionalproteinprocessesafterthetranscription,translationregulationprocessing,controlingtoformandsoonsteps.Thusthedirectstudyofproteintotheinterpretation(翻译)oftherealli

3、fephenomenonisofgreatimprotance.Butresearchonmeansandmethods(手段方法)oftheproteindoesn'thaveabigdevelopment,sothesearchforeffectiveandfastproteinanalysistechnologyhasbecomeoneofthemostimportantstep.ThencomestheproteinchipTheproteinchip（蛋白芯片）technologyisquic

4、klydevelopedinrecentyearsinchemicalbiologyasahighandnewtechnology.Itisthebasicprincipleismakevariousproteinsorderlyfixedonallkindsofmediumcarrier（介质载体）asatestchip.Andthen,usingthetagantibodies(抗体)thatarespecifiedbythefluorescent(荧光)substancematchwiththepr

5、otein.Andthecorresponding(对应的)proteinwillmatchwiththechip.WhatisProteinmicrochip???Then,thefluorescentontheantibodywillindicatethecorresponding(对应的)numberofproteinanditsexpression.Alltheotherantibodiesthatdoesn'tmakecomplementarycombinationwillbewashedaft

6、erusingthefluorescentscanner(荧光扫描仪)orlaserscanningtechnology(激光扫描共聚技术).Throughanalysisthefluorescenceintensity(强度)ofeachpointonthechipandinteractionrelationshipbetweenproteinandprotein,thepurposeofachievingdeterminingallsortsoffunctionofgeneexpressionwill

7、bedone.Therearethreetypesofproteinmicroarraysthatarecurrentlyusedtostudythebiochemicalactivitiesofproteins.AlltypesofchipsAnalyticalmicroarraysarealsoknownascapturearrays.Inthistechnique,alibraryofantibodies,aptamersoraffibodiesisarrayedonthesupportsurfac

8、e.Theseareusedascapturemoleculessinceeachbindsspecificallytoaparticularprotein.Thearrayisprobedwithacomplexproteinsolutionsuchasacelllysate.Analysisoftheresultingbindingreactionsusingvariousdetectionsystemscanprovid