应技大基因工程200道选择题.doc

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1、AThevarioustechniquesbywhichscientistsmanipulateDNAinthelabarecalledA.geneticengineeringB.biotechnologyC.recombinantDNAD.gelelectrophoresisE.geneprobesDThefunctionofthedideoxy(dd)nucleotidesthatareusedintheSangermethodofDNAsequencingistoA.denatureDNAintosinglestrandsBserveasprimersCbe

2、afluorescenttagD.incorporateintonewlyreplicatedDNAstrandsandstopelongation.纳入新复制的DNA链,停止伸长。E.noneBWhichtypeofmutationleadestoaframeshiftmutation?A.missenseB.deletion缺失C.nonsenseD.BasesubstitutionE.AllBThermococcuslitoralisandThermusaquaticusarethermophilicbacteriathatareA.usedasclonin

3、gvectors.B.sourcesofheatstableDNApolymerases.热源稳定DNA聚合酶。C.geneticallyengineeredbacteriaD.principalsourcesofretrictionendonucleasesE.NoneEThesitewheretheoldDNAstrandsseparateandnewDNAstrandswillbesynthesizediscalledtheA.primerB.OkazakifragmentC.templateDrollingcircleE.replicationfork复制

4、叉BEnzymesthatcatalyzeremovingelectronsfromonesubstractandaddingelectronstoanotherarecalled:A.phosphotranferasesB.oxidoreductases.氧化还原酶C.decarboxylasesD.aminotransferasesE.ligasesASequencesofDNAthatareidenticalwhenreadfromthe5'to3'directionononestrandand3'to5'directionotheotherstrandar

5、eApalindromes回文BreversetranscriptaseCrestrictionendonucleasesDLigasesEDNApolymerasesDThecommercialproductFrostbancontainsageneticallyengineeredstrainofA.EscherichiacoliB.SaccharomycescerevisiaeC.ThermusaquaticusD.Pseudomonassyringae丁香假单胞菌E.PseudomonasfluorescensCAllofthefollowingareco

6、rrectaboutDNAfingerprinting,exceptA.itdisplaystheuniquepatternofanindividualsDNAfragmentsB.itcanbeusedtoanalyzeevidencewhenonlyminuteamountsofDNAarefoundC.itcannotbeusedonspecimensolderthan5years它不能被用于标本5年以上D.itisroutinetouseitastrialevidenceintheUSE.itcanbeusedonhairandsalivaDThisiso

7、ftenusedinforensicsciencetodistinguishonesequenceofDNAfromanotherbycomparingthesequenceofthestrandsatspecificloci:A.cloningB.genetherapyC.antisensetherapeuticD.DNAfingerprintingDNA指纹分析E.noneBWhichofthefollowingmethodsdetermineswhichgenesareactivelytranscribedinacellunderavarietyofcond

8、ition