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1、Affymetrix基因芯片产品Upto~7,000,000features/chip5-11µmRNAtargetThousandsofcopiesofaspecificoligonucleotideprobeineachfeatureRawdataimagewith1-6.9milliondifferentcomplementaryprobesOligonucleotideprobe*****1.28cmHybridizedprobecell*5”5”芯片产品分类表达谱芯片-mRNA3’IVTWTMicroRNA芯片lncRNA
2、芯片SNP/CNV芯片甲基化&转录因子芯片表达谱芯片3’IVTHumanU133Plus2.0MouseU4302.0RatU2302.0PrimeView&AlmacXcelWTHuman/Rat/MouseGene1.0STHuman/Rat/MouseExon1.0STHTA2.0表达谱芯片探针设计Exon1.0ST基因组DNA初始转录本RNA可变剪切转录本Gene1.0ST3’端表达谱表达谱芯片实验步骤样本RNA抽提&质检cDNA合成及片段化生物素标记芯片杂交-洗脱-扫描芯片数据标准化Gene系列芯片Transcriptco
3、verageofthearrayHumanGene1.0STArrayHumanGene2.0STArrayTotalRefSeqtranscriptscovered36,07940,716NM?RefSeqcodingtranscript, well-establishedannotation32,02030,654NR?RefSeqnon-codingtranscript, wellestablishedannotation2,9675,638XM?RefSeqcodingtranscript, provisionalan
4、notation579996XR?RefSeqnon-codingtranscript,provisionalannotation5133,428lincRNAtranscripts46611,086RefSeq(Entrez)genecount21,01424,838EXON系列芯片DesignStatisticsSummaryProbeSets1.4millionExonClusters>1millionSupportedbyPutativeFull-LengthmRNA289,961probesetsSupportedbyEn
5、semblTranscripts306,583probesetsSupportedbyEST665,175probesetsSupportedbyMouseorRatmRNA220,262probesetsSupportedbyGenePrediction883,105probesetsProbeSelectionRegionAlongtheentirelengthofthetranscriptsProbes/ProbeSelectionRegion4BackgroundSubtractionStrategyMedianintens
6、ityofupto1,000backgroundprobeswiththesameGCcontentTotalFeaturesPerArray>5,500,000InterrogatedStrandSense²HTA2ArraycontentProteincodingcontentNon-proteincodingcontentGenes(transcriptclusters)44,69922,829Transcripts245,34940,914Exons560,472109,930Exonclusters296,05882,44
7、4Splicejunctions(probesets)339,1460表达谱芯片应用U133——经典，相对旧，无价格优势，不做推荐Gene系列——基本，仅满足一般表达量检测需求Exon系列——全面，精确表达量检测HTA2——最佳，最全&最精确表达量EG1.0——最佳，唯一能够获得转录本表达量MicroRNA芯片MicroRNA4.0microRNAPre-microRNAsnoRNA&scaRNAmicroRNA芯片实验步骤样本RNA抽提&质检Poly(A)加尾&FlashTagBiotinHSR连接生物素标记芯片杂交-洗脱-扫描芯
8、片数据标准化microRNA4.0特点100%覆盖miRBaseV20包括所有数据库记录的物种人2578小鼠1908大鼠728重复性>0.95技术重复生物重复lncRNA芯片Human/Mouse/RatGene2.0HTA2.0(H
