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1、中国妇产科临床杂志2017年1月第18卷第1期 ChinJClinObstetGynecol January 2017,Vol.18,No.1●17●·论著·HPV16基因整合与FHIT蛋白缺失在宫颈癌变中的作用徐又先袁林濮德敏马丁【摘要】目的探讨HPV16的基因型整合状态及FHIT基因缺失在宫颈癌发生发展中的作用及相关性。方法选取44例宫颈癌、58例宫颈上皮内瘤样病变(CIN)和20例宫颈正常组织的患者,免疫组化检测宫颈FHIT蛋白的表达;并用多重PCR法检测HPV16E2/E7表达。结果122例单一
2、HPV16阳性标本HPV16总整合率为78.69%,在正常宫颈组织、CIN1、CIN2、CIN3和宫颈癌中整合率分别为60.00%、66.67%、75.00%、86.96%和88.63%,随病变加重,整合率增强,组间差异有统计学意义(P<0.05);FHIT蛋白的总缺失率为43.44%,FHIT蛋白在正常宫颈组织、CIN1、CIN2、CIN3和宫颈癌中的缺失率分别为15.00%、20.00%、25.00%、43.48%和72.73%。FHIT蛋白的缺失率,随病变加重,缺失率增高,组间差异有统计学意义(P
3、<0.05)。FHIT蛋白缺失在不同HPV16整合时不同,差异有统计学意义(P<0.05)。结论HPV16基因整合和FHIT蛋白缺失在宫颈癌的发生、发展中起重要作用,HPV16基因整合可能通过诱导FHIT基因低表达从而促使宫颈癌发生发展。【关键词】人乳头状瘤病毒;脆性组氨酸三联体;整合;宫颈上皮内瘤样病变;宫颈癌Theintegrationofhumanpapillomaviral16anditsrelationshipwithFHITexpressionincervicalcancerXUYouxia
4、n,YUANlin,PUDemin,MADing.(DepartmentofObstetrics&Gynecology,theFifthHospitalofWuhan,HubeiWuhan430050,China)【Abstract】ObjectiveToexploretheintegrationofhumanpapillomaviral16(HPV16)anditsrelationshipwiththeexpressionoffragilehistidinetriad(FHIT)incervicalc
5、ancer.MethodsThephysicalstatusofHPV16E2/E7wasdetectedbyMultiplexPCR(Polymerasechainreaction)in44patientswithcervicalcancer,58patientswithCINand20patientswithnormalcervixtissues.AndimmunohistochemistryS-Passaywasusedtodetecttheex-pressionofFHITbeforeopera
6、tion.ResultsThetotalintegrationrateofHPV16was78.69%in122cervicalspecimenswithonlyHPV16positive.TheintegrationratesofHPV16were60.00%,66.67%,75.00%,86.96%and88.63%,innormalcervixtissues,CIN1,CIN2,CIN3andcervicalcancer.Anditshowed2anincreasingtrendindiffere
7、ntcervicallesions(χ=5.317P=0.003<0.05);ThetotaldescendingrateofFHITwas43.44%,thedescendingratesofFHITwere15.00%,20.00%,25.00%,43.48%and72.73%,respectivelyindifferentcervicallesions.The2differencewasstatisticallysignificant(χ=9.931P=0.002<0.05).Thediffere
8、ncebetweenthetotalintegrationofHPV16andthe2totaldescendingexpressionofFHITwassiginificant(χ=11.211P=0.002,KruskalWallistest).ConclusionThereisacloserelationshipbetweenHPV16genomeintegrationcorrelatesandthedescendingexpress