《lorenz-mielightscatteringincellularbiology》.pdf

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1、Gen.Physiol.Biophys.(1992),11,133—151133Lorenz-MieLightScatteringinCellularBiologyJ.ULIČNÝDepartmentofNuclearPhysicsandBiophysics,FacultyofSciences,P.J.ŠafárikUniversity,Jesenná5,04154Košice,CzechoslovakiaAbstract.TheLorenz-Mielightscatteringisdiscussedasatoolallowinglivingcellchara

2、cterization.Thescatteredlightcarriesinformationaboutthesize,shape,internalstructureandrefractiveindexofthecell.Theadvantagesoflightscatteringmethodsconsistinhighspeed,nondestructive,sensitiveandrelativelyeasymeasurements.Lightscatteringmethodsarecompatiblewithothermethods.Inlightsca

3、tteringinbothstaticandflowsystems.Forsphere-likecellsreliablesizeandrefractiveindexinformationcanbeextracted.Ontheempiricalbasis,lightscatteringpatterncanbeusedforthecellidentificationandseparationpurposes.Thefullutilizationofthelightscatteringinformationislimitedduetothelackoftheor

4、eticalknowledgeaboutthecomplexscattererpropertiesandefficientinversionschemes.Therapidprogressincomputertechniqueandinsingle-particlescatteringexperimentsmaysignificantlyimprovetheinterpretationoflightscatteringpatternsofthebiologicalparticles.Keywords:Lightscattering—Livingcellchar

5、acterization—Sizedetermination—Lorenz-MiescatteringIntroductionTheinterestinusingLorenz-Mielightscattering(LMLS)incellularbiologyhasbeenlastingformorethantwentyyears.Therapiddevelopmentofthephotonics(laserlightsources,lightdetectors)andelectronics(dataacquisitionelectronics,laborato

6、rycomputers)havemadethelightscattering(LS)techniquesmoreavailableinlaboratories,thebiologicalonesincluded.However,besidesthetechnicalequipmentthelackofinformationaboutthecapabilitiesandlimitationsofLStechniqueshaspreventedtheirwiderutilization.Thepresentarticlediscussesthepotentials

7、andlimitationsofLMLStechniquesasasourceofinformationaboutbiologicalparticles,especiallyintactlivingcells.134UličnýTheoryoflightscatteringbysmallparticlesThetermLorenz-Miescatteringdenoteselasticlightscatteringbysmallparticles.WepreferthistermtothatofRayleighscatteringbecauseoftheamb

8、iguitiesconnectedwi