分析测试高毅.ppt

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1、Apyrene-basedhighlyselectiveturn-onfluorescentchemosensorforIron(III)ionsanditsapplicationinlivingcellimaging作者：PeterKunChung&Shi-RongLiu&Hsuan-FuWang&Shu-PaoWu来源：JOURNALOFFLUORESCENCE杂志简介：Journaloffluorescence(荧光杂志)主要发表最新的荧光方面研究成果，包括荧光研究方面的进展理论，数据分析和表征等。影响因子：2012年度（2.107）2011年度（

2、1.789）Abstract：Anewpyrene-basedchemosensor(1)exhibitsexcellentselectivityforFe3+ionsoverawiderangeoftestedmetalionsAg+,Ca2+,Cd2+,Co2+,Cu2+,Fe2+,Hg2+,K+,Mg2+,Mn2+,Ni2+,Pb2+,andZn2+.ThebindingofFe3+tochemosensor1producesanemissionbandat507nmduetotheformationofaPy-Py*excimerthatisin

3、ducedbyFe3+-binding.Inaddition,fluorescencemicroscopyexperimentsshowthatchemosensor（1）canbeusedasafluorescentprobefordetectingFe3+inlivingcells实验仪器8453UV/Visspectrometer(Agilent,USA)DA8.3Fourier-TransformInfraredSpectrometer(Bomem,ABB,Swiss)ZEISSAxioScopeA1FluorescenceMicroscope(

4、ZEISS,Germany)实验部分1、SynthesisofChemosensor12、MetalionBindingStudybyUV–visandFluorescenceSpectroscopyChemosensor1(2μM)wasaddedwithdifferentmetalions(20μM).Allspectraweremeasuredin1.0mLacetonitrile-acetonesolution(v/v=99:1).结果与讨论：Fig.1AbsorptionchangestovariousequivalentsofFe3+inCH

5、3CN/acetone(v/v=99:1)solutions.Theexcitationwavelengthwas382nm1、紫外可见光谱2、荧光光谱3、红外光谱结论Theexperimentsynthesizechemosensor1fromthereactionof1-pyrenecarbox-aldehydehydrazoneand1,4-bis(2-formylphenoxy)butanetoformtwoiminebondsbetween1,4-bis-phenoxybutaneandpyrene.Weobservedsignificantf

6、luorescenceenhancementwithchemosensor1inthepresenceofFe3+.Thankyou