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1、45.4.06C(i)High-performanceanion-exchangechromatographAOACOfficialMethod2000.11(HPAEC–ED).—LCcapableofproducingca3000psi(200bar);PolydextroseinFoodsgradientpump(capableofhandlingNaOHeluents),andpulsedinte-IonChromotographygratedelectrochemicaldetecto
2、r(withgoldelectrode),e.g.,DionexFirstAction2000Corp.(POBox3603,Sunnyvale,CA94088-3603,USA)DX500[Applicabletothedeterminationof2–95%(w/w)polydextroseinbasicgradientcarbohydratesystem,orequivalent.SeeTa-foods.]ble2000.11BfordetectorvoltagesettingsforDi
3、onexED40.SeeTable2000.11Afortheresultsoftheinterlaboratorystudy(j)LCcolumn.—Microporoussubstrate(10mm)agglomeratedsupportingacceptanceofthemethod.withmicrobeadquaternaryammoniumfunctionalizedlatex,250´4mmcolumn[e.g.,CarboPakPA1(Dionex,orequivalent)]w
4、ithA.Principleguardcolumn(e.g.,CarboPakPA1Guard,23´3mm,orequivalent).Polydextroseisextractedfromfoodwithhotwaterandcentri-fuged.Thesupernatantpassesthroughacentrifugalultrafiltertore-C.Reagentsmovehighmolecularweightinterferences.Thefiltrateistreated
5、(a)Solvents.—Deionizedwater(resistance³18MS-cm).withanenzymemixture(isoamylase,amyloglucosidase,and(b)Sodiumhydroxide.—50%;carbonatefree,density1.54kg/L.fructanase)toremoveanyoligosaccharideinterferences,mainlyTo100gNaOH,containing£1%Na2CO3,add100mLH
6、2O.Stop-malto-oligomersandfructans.Polydextrosestandardsundergotheperandswirluntilsolutioniscomplete.LetstanduntilNa2CO3hassametreatment.High-pressureanionexchangechromatographysettled,leavingclearliquid(about10days).Keeptightlyclosedwithelectrochemi
7、caldetection(HPAEC–ED)isusedtoquantitateawhennotinuse.highmolecularweightfractionofpolydextrose.Thispolydextrose(c)Aceticacid.—0.2M.Dilute1.20gaceticacidto100mLwithmethodcanbeusedasanadjuncttothecurrentAOACmethodsforwater.measuringtotaldietaryfiber.(
8、d)Sodiumacetatesolution.—0.2M.Dissolve2.72gNaace-B.Apparatustate×3H2Oinwateranddiluteto100mL.(a)Waterbaths.—Setat80±2°C(increasedtoboiling);and50±(e)Acetatebuffer.—pH4.5.Mix28mLaceticacid,(c),with2°C.22mLsodiumacetate,(d),anddiluteto100mLwithwater.(b
