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1、植物牛态学报200&32(5)1002-1006JournalofPlantEcology(ChineseVersion)He・Ne激光处理与干旱胁迫对小麦幼苗保护酶活性及脂质过氧化作用的影响邱宗波阮刘晓
2、李方民
3、田向军
4、岳明广(1西部资源生物与现代生物技术省部共建教育部童点实验穿(西北大学),西安710069)(2河南帅范大学生命科学学院,河南新乡453007)摘要用He・Ne激光(5.23mW・mm3处理经5%、10%、15%PEG6000胁迫的小麦幼苗,分析「干旱胁迫条件下激光处理对小麦幼苗保护酶活性及脂质过氧化作用的影响。适度干早胁迫的
5、小麦幼苗经He・Ne激光辐照后,丙二醉(MDA)含暈和超氧自由基(O27)产生速率显著降低(p<0.05),而过氧化物酶(POD)活性和抗坏血酸(AsA)、谷胱甘肽(GSH)含最却显苦增加(p<0.05).总体上看,5%和10%PEG6000胁迫的小麦幼苗经激光辐照3min后抗早性增强.关键词He・Ne激光小麦干早胁迫保护酶脂质过氧化INFLUENCEOFHe-NeLASERIRRADIATIONONPROTECTIVEENZYMEACTIVITIESANDLIPIDPEROXIDATIONINWHEATSEEDLINGSBYDROUGHTST
6、RESSDAMAGEQIUZong-Bou,LIUXiao1,LIFang-Min1,TIANXiang-Jun1,andYUEMin尹iKeyLaboratoryofResourcesBiologyandBiotechnologyinWesternChina(NorthwestUniversity),MinistryofEducation.Xi'em710069.China,and2CollegeofLifeSciences.HenanNormalUniversity,Xinxiang.Henan453007.ChinaAbstractAim
7、sWestudiedprotectiveenzymeactivitiesandlipidperoxidationinwheatseedlingstreatedwithdifferentconcentrationofPEG6000todeterminetheeffectsofHe-Nelaseronwheat(Triticumaestivuni)exposedtodroughtstress.MethodsWetreated12-day-old,drought-stressedseedlingswith5%,10%and15%PEG6000for3
8、,6,9,12and15days.After3,6,9,12and15daysofdroughtstress,aHe・Nelaserwasemployedtoirradiateseedlingsofspringwheatfor0,1and3min.Leavesweresampledforvariousanalyses.ImportantfindingsHe-Nelaserirradiationsignificantlydecreasedmalondialdehyde(MDA)contentandsuperoxideradical(O2Jpro
9、ductionrateandnotablyincreasedperoxidase(POD)enzymeactivityandascorbate(AsA)andglutathione(GSH)concentrationinwheatseedlingsexposedtomoderatedroughtstress.Weconcludedthat3minlaserirradiationenhancedanti-droughtcharacteristicsofwheatseedlingstreatedwith5%or10%PEG6000.Keywords
10、He-Nelaser,wheat,droughtstress,protectiveenzyme,lipidperoxidationDOI:10・3773"・issn」005-264x.2008.05.004适当剂量的激光辐射可以提高种子的萌发率、提高酶的活性、叶绿素含量以及植物的抗逆性(蔡素雯等,2000)。韩榕等(2002)报道,用He・Ne激光辐照小麦(Triticumaestivuni)可提高其酶活性,对细胞膜损伤具有修复作用,增强抗逆性。Qi等(2000,2002)发现He-Ne激光辐射蚕豆(Vicia沧巾)幼苗可提高SOD、POD、C
11、AT酶活性,改变SOD、CAT同工酶谱从而对UV・B辐射损伤具有一收稿H期:2007-02-08接受H期:2007-04-26基金项目:国家白然科学基