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1、ProteinPurificationHandbook18-1132-29EditionABHiTrap,Sepharose,STREAMLINE,Sephadex,MonoBeads,MonoQ,MonoS,MiniBeads,RESOURCE,SOURCE,Superdex,Superose,HisTrap,HiLoad,HiPrep,INdEX,BPG,BioProcess,FineLINE,MabTrap,MAbAssistant,Multiphor,FPLC,PhastSystemandÄKTAaretrademarksofAmershamPharmaciaB
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3、opyofthesetermsandconditionsofsaleisavailableonrequest.©AmershamPharmaciaBiotechAB1999-Allrightsreserved.AmershamPharmaciaBiotechABSE-75184UppsalaSwedenAmershamPharmaciaBiotechUKLimitedAmershamPlaceLittleChalfontBuckinghamshireEnglandHP79NAAmershamPharmaciaBiotechInc800CentennialAvenuePO
4、Box1327PiscatawayNJ08855USAProteinPurificationHandbookContentsIntroduction........................................................................................................7Chapter1PurificationStrategies-ASimpleApproach......................................................9Preparat
5、ion............................................................................................10ThreePhasePurificationStrategy..........................................................10GeneralGuidelinesforProteinPurification............................................12Chapter2Preparat
6、ion......................................................................................................13BeforeYouStart....................................................................................13SampleExtractionandClarification.................................................
7、.....16Chapter3ThreePhasePurificationStrategy....................................................................19Principles................................................................................................19SelectionandCombinationofPurificationTechniques..
