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1、浙江省"浙八味"道地药材真菌病害调查及病原鉴定■农学论文浙江省〃浙八味〃道地药材真菌病害调查及病原鉴走钱永生，蔡苏，吴剑丙，冒佩佩，马婉琴，楼锂函，王慧中（杭州师范大学生命与环境科学学院,杭州310036）摘要：通过对浙江省磐安地区〃浙八味〃药材真菌病害进行病原菌分离和鉴定，采用无菌培养基平板分离技术,从药材植物病叶中分离获得病原真菌，构建真菌ITS系统发育树,对病原菌进行分子水平的分类鉴定,并观察病原菌菌落形态及显微结构和形态学鉴定共获得9种病原真菌，分别为链格孑包菌（Alternariasp尖孑包镰刀菌（Fusariumoxysporum球毛壳菌（Chae
2、tomiunnglobosuml绿色木霉菌（Hypocreavirens>月状旋抱腔菌（Cochliobolus1un□□atusX球抱黑抱霉（NigrosporasphaericaX木贼镰刀菌（FusariumequisetiX束状刺盘孑包（Co11etotrichumdematium）和茎点霉菌（Phomasp.L查阅药用植物病原菌记录并结合科赫法则实验，当地药用植物成为3种病原菌的新寄主，分别为月状旋抱腔菌引起的安徽亳州白芍褐斑病、束状刺盘胞引起的磐安阔叶麦冬炭疽病、球毛壳菌引起的早小洋菊叶枯病。关键词：〃浙八味〃；中药材；病原真菌；ITS;鉴定中图分类号
3、：S432文献标识码：A文章编号：0439・8114（2015）19・4740-06D01:10.14088/j.cnki.issn0439-8114.2015.19.021InvestigationandIdentificationofthePathogenicFungiinTrueborn"Zhe・ba・weiz/MedicinalHerbsinZhejiangProvinceQIANYong-sheng,CAISu,WUJian・bing,MAOPei-pei,MAWan・qin,LOUYu-han,WANGHui-zhong(CollegeofLifea
4、ndEnvironmentSciences,HangzhouNormalUniversity,Hangzhou310036,China)Abstract:Thepathogenspeciesandinfectionstatusintrueborn"Zhe・ba・weiz/medicinalherbsweredeterminedthroughinvestigationandidentificationofthepathogenicfungiinPan'anregionofZhejiangprovincezshou1dbeprovidedtheguideforpro
5、tectmedica1herbsp1antinginlargescaleandeco-friendcondition.Pathogenicfungistrainswereseparatedfromtheinfectedleavesofmedicinalherbsbyplateseparationinfertilemedium.ThesepathogenswereidentifiedbyconstructionoffungalITSphylogenetictree,andthenanalysisofcolonialmorphologyandmicrostructu
6、re.9typesofpathogenicfungiwerefinallyobtained,namelyAlternariasp.zFusariumoxysporum,Chaetomiumglobosum,Hypocreavirens,Cochlioboluslunatus,Niporasphaerica,FusariumequiseColletotrichumdematium,ComparedwiththerelatedrKoch1spostulates,the1ocherbsbecamenewhostsforpathogenes.Inthesepathog1
7、iobolus1unatuswastheentofbrownspotdiseasesinWheonyRoot'AnhuiColletotrhumdematiuminducedanthracnoseiiriopeplatyphylla'Pan7an'andChatomiumglobosumcausedtheleafb1ightofChrysanthemum'Zaoxiaoyangju'・Keywords:nZhe・ba・weiz/;medicinalherbs;pathogenicfungi;ITS;identification收稿日期:2015基金项目:浙江省教
8、育厅项目（Y201431
