欢迎来到天天文库
浏览记录
ID:45564580
大小:246.15 KB
页数:14页
时间:2019-11-14
《Ajax应用安全研究》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、AFMSpectroscopyME382:Micro/NanoScienceandEngineeringRaviAgrawal,KevinLee,andDeepakPonnavoluReportSubmitted:03/12/05PresentedtoProfessorABSTRACTInthispaper,wediscusstheuseofanAtomicForceMicroscope(AFM)todospectroscopy.SpectroscopyofDNA,proteinsandotherbiomoleculesareimportant
2、inordertocharacterizethebiomoleculeaswellasdetermineitsproperties.SomemajorconsiderationsforspectroscopyincludetheuseofanAFMinliquidsforusewithbiomoleculesandthefunctionalizationofsubstrateandthetipforalignmentandselectivity.Potentialapplicationsforforcespectroscopyincludech
3、aracterizationofsinglemoleculesundertensileortorsionalload,cell-cellinteractions,andinteractionofsurfaceswithmoleculesetc.AhugeamountofresearchisbeingdoneinthisareatoexploittheresolutionobtainedthroughAFMsinthesekindsofapplications.CONTENTS1.INTRODUCTION22.WORKINGPRINCIPLE23
4、.USINGAFMINAQUEOUSMEDIUM34.FUNCTIONALIZATIONOFSUBSTRATE45.TIPPARAMETERSANDTOPFUNCTIONALIZATION66.APPLICATIONSANDDEVELOPMENTS107.CONCLUSION111.INTRODUCTIONSpectroscopyistypicallyusedinthephysicalandanalyticalchemistryfortheindentificationofsubstances.InvolvingtheuseoftheAtomi
5、cForceMicroscope,forcespectroscopyisadynamicanalyticaltechniquewhichallowsthestudyofmechanicalpropertiesofpolymermoleculesandchemicalbonds-Forcespectroscopymeasuresthebehaviorofamoleculeunderstretchingortorsionalforces,andunlikemostotherformsofspectroscopydoesnotmeasurethema
6、tterradiationinteraction.2.WORKINGPRINCIPLEAFMSpectroscopyisbasedonthesimpleprincipleofattachingthetipofthecantilevertooneendofthebiomoleculestobeanalyzed,theotherendbeingheldbythesubstrate.Thecantileveristhenmovedusingapiezocontrollertoexertatensileloadonthemolecule.Usingt
7、heForcemodeofAFM,forcecharacteristiccurvesforthebiomoleculescanbeobtainedinthismanner.Fig.2」showsaschematicviewoftheprocess-SFMcantileverLigand/ReceptorinteractionsProteinunfoldingLigandFig.2.1SchematicviewofsinglemoleculeForceSpectroscopyusingligand-receptorbinding38、MINAQUEOUSMEDIUMAtomicForceMicroscopy(AFM)isamethodinwhichaprobescansthesur
8、MINAQUEOUSMEDIUMAtomicForceMicroscopy(AFM)isamethodinwhichaprobescansthesur
此文档下载收益归作者所有