Ajax应用安全研究

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1、AFMSpectroscopyME382:Micro/NanoScienceandEngineeringRaviAgrawal,KevinLee,andDeepakPonnavoluReportSubmitted:03/12/05PresentedtoProfessorABSTRACTInthispaper,wediscusstheuseofanAtomicForceMicroscope(AFM)todospectroscopy.SpectroscopyofDNA,proteinsandotherbiomoleculesareimportant

2、inordertocharacterizethebiomoleculeaswellasdetermineitsproperties.SomemajorconsiderationsforspectroscopyincludetheuseofanAFMinliquidsforusewithbiomoleculesandthefunctionalizationofsubstrateandthetipforalignmentandselectivity.Potentialapplicationsforforcespectroscopyincludech

3、aracterizationofsinglemoleculesundertensileortorsionalload,cell-cellinteractions,andinteractionofsurfaceswithmoleculesetc.AhugeamountofresearchisbeingdoneinthisareatoexploittheresolutionobtainedthroughAFMsinthesekindsofapplications.CONTENTS1.INTRODUCTION22.WORKINGPRINCIPLE23

4、.USINGAFMINAQUEOUSMEDIUM34.FUNCTIONALIZATIONOFSUBSTRATE45.TIPPARAMETERSANDTOPFUNCTIONALIZATION66.APPLICATIONSANDDEVELOPMENTS107.CONCLUSION111.INTRODUCTIONSpectroscopyistypicallyusedinthephysicalandanalyticalchemistryfortheindentificationofsubstances.InvolvingtheuseoftheAtomi

5、cForceMicroscope,forcespectroscopyisadynamicanalyticaltechniquewhichallowsthestudyofmechanicalpropertiesofpolymermoleculesandchemicalbonds-Forcespectroscopymeasuresthebehaviorofamoleculeunderstretchingortorsionalforces,andunlikemostotherformsofspectroscopydoesnotmeasurethema

6、tterradiationinteraction.2.WORKINGPRINCIPLEAFMSpectroscopyisbasedonthesimpleprincipleofattachingthetipofthecantilevertooneendofthebiomoleculestobeanalyzed,theotherendbeingheldbythesubstrate.Thecantileveristhenmovedusingapiezocontrollertoexertatensileloadonthemolecule.Usingt

7、heForcemodeofAFM,forcecharacteristiccurvesforthebiomoleculescanbeobtainedinthismanner.Fig.2」showsaschematicviewoftheprocess-SFMcantileverLigand/ReceptorinteractionsProteinunfoldingLigandFig.2.1SchematicviewofsinglemoleculeForceSpectroscopyusingligand-receptorbinding3

8、MINAQUEOUSMEDIUMAtomicForceMicroscopy(AFM)isamethodinwhichaprobescansthesur