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1、第29卷第1期环境科学学报Vol.29,No.12009年1月ActaScientiaeCircumstantiaeJan.,2009朱晓宇,王世梅,梁剑茹,等.2009.两株高效好氧反硝化细菌的分离鉴定及其脱氮效率[J].环境科学学报,29(1):111-117ZhuXY,WangSM,LiangJR,etal.2009.IsolationandidentificationoftwoaerobicdenitrifierswithhighefficiencyintheremovalofNfromsimulatedwastewater[J
2、].ActaScientiaeCircumstantiae,29(1):111-117两株高效好氧反硝化细菌的分离鉴定及其脱氮效率3朱晓宇,王世梅,梁剑茹,周立祥南京农业大学资源环境科学学院环境工程系,南京210095收稿日期:2008202201录用日期:2008210208摘要:从水稻土和活性污泥中分离到两株可以在好氧条件下进行反硝化作用的细菌ZW23和ZW27.通过对这两株细菌的形态观察和生理生化特征,以及16SrDNA序列测定认为菌株ZW23和ZW27分别是假单胞菌属类产碱杆菌(Pseudomonaspseudoalcalige
3、nes)和假单胞菌属门多萨菌(Pseudomonasmendocina).好氧培养条件下,在初始氮源约为280.00mg·L-1的反应体系中,两株细菌在12h内均引起体系中总氮显著下降,削-1-1-1-1减率分别达66.43%和65.54%,其余总氮几乎全部转化为内源氮.脱氮速率分别达到约21.72mg·L·h和22.31mg·L·h,比现已分离出的兼性好氧反硝化细菌的脱氮速率要快得多.反应过程中没有检测到亚硝态氮和氨态氮的积累.菌株ZW23和ZW27是两株典型的高效兼性好氧反硝化细菌,具有重要的应用价值.关键词:细菌;好氧反硝化;分离
4、;鉴定;脱氮效率文章编号:025322468(2009)012111207中图分类号:X172文献标识码:AIsolationandidentificationoftwoaerobicdenitrifierswithhighefficiencyintheremovalofNfromsimulatedwastewater3ZHUXiaoyu,WANGShimei,LIANGJianru,ZHOULixiangDepartmentofEnvironmentalEngineering,CollegeofResourcesandEnvironm
5、entalSciences,NanjingAgriculturalUniversity,Nanjing210095Received1February2008;accepted8October2008Abstract:Twostrainsofaerobicdenitrifierswereisolatedfromfloodedpaddysoilandactivesludge,respectively.Thetwostrains,codedasZW23andZW27,arefacultativebacteria.Inthelightofthe
6、irmorphologicalandphysiologicalfeaturesaswellastheir16SrDNAsequences,theywereidentifiedasPseudomonaspseudoalcaligenesandPseudomonasmendocina.Underaerobicincubationconditions,NlossthroughdenitrificationintheliquidculturemediuminoculatedwithZW23andZW27reached66.43%and65.54
7、%in12hours,respectively.TheircorrespondingNremovalrateswere21.72-1-1-1-1mg·L·hand22.31mg·L·h,whichweremuchhigherthanthosepreviouslyreported.Almostallofthenitrogen,otherthantheNlostthroughdenitrification,wasassimilatedbythedenitrifiers.Nitriteandammoniawerenotobservedduri
8、ngtheincubation.Inconclusion,strainsZW23andZW27aretwotypicalfacultativeaerobicdenitrifiersofhighefficie
