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1、中医辨证治疗对NAFLD大鼠血脂等影响实验探究摘要：目的：探讨中医辨证治疗对非酒精性脂肪性肝病（NAFLD）大鼠各时期血脂等指标影响的作用机制，为临床防治NAFLD提供理论依据。方法：取SPF级Wistar大鼠72只，雌雄各半，随机分为正常组、模型组、对照药物治疗组、药物A方治疗组、药物B方治疗组、先A方后B方治疗组、先E方后A方治疗组、先空白后A方治疗组、先空白后B方治疗组，共9组。除正常组给予普通饲料外，其余各组均饲以高脂饲料。造模第4周后，模型组加用生理盐水灌胃；对照组加用对照药物灌胃；药物A方组加用治疗药物A方灌胃；药物B方组加用治
2、疗药物B方灌胃；先A方后B方治疗组即4~8周用A方，旷12周用B方灌胃；先B方后A方治疗组即4~8周用E方，旷12周用A方灌胃；先空白后A方治疗组即先空白，第8^12周用A方灌先空白后B方治疗组即先空白，第8~12周用B方灌胃。实验第12周时，测定大鼠血清TCH、TG、HDL、LDL、FFA、GSH—ST、TNF-a的值。结果:实验第12周时，先A方后B方治疗组（编号为6组）降低NAFLD后期大鼠血清TG、TCH、LDL、FFA、TNF-a及升高血清GSH-ST水平的疗效与其他治疗组相比尤为显著（P〈0.01或P<0.05）o结论：在NAF
3、LD的整个病程中，采取早期阶段运用疏肝健脾、活血化瘀、化痰利湿功效的治法，后期阶段运用化痰消瘀、清热利湿、补益肝肾功效的治法的治疗方案，对于降低大鼠血脂等指标及提髙大鼠抗氧自由基的疗效显著优于其他治疗措施。关键词:中医多法治疗；血脂；NAFLD；TNF-a；GSH-ST❷中图分类号：R~33文献标识码：A文章编号：1673-7717(2008)11-2402-04XExperimentalStudyonEffectofTraditionalChineseMedicineDialecticalTreating❷Blood-fatIndexon
4、NAFLDRatsLIYun❷cheng❷1,SHENGGuo❷guang❷2,HUANGYu❷hug2,XUJian❷liang❷2❷(1.HubeiCollegeofTraditionalChineseMedicine,Wuhan430061,Hubei,China；❷2.InstituteofLiverDisease,HubeiProvincialHospitalofTraditionalChineseMedicine,Wuhan430061,Hubei,ChinaAbstract:Objective:Toexplorethefunc
5、tionmechanismoftraditionalChinesemedicinedialecticaltreatingblood-fatindexandsooninnonalcoholicfattyliverdisease(NAFLD)rats，varioustimes,whichprovidethebasistheorytopreventandcuretheNAFLDinclinic.Methods：Fetched72wistarrats'levelofSPF,femalesandmaleswerehalfandhalf,werediv
6、idedinto9groupsatrandom：Normalgroup,Modelgroup,Comparisonmedicinetreatmentgroup,MedicineAtreatmentgroup,MedicineBtreatmentgroup,FirstMedicineAthenMedicineBtreatmentgroup,FirstMedicineBthenMedicineAtreatmentgroup,FirstblankthenMedicineAtreatmentgroup,FirstblankthenMedicineB
7、treatmentgroup・Exceptthenormalgroupwasfedordinarydiet,theothereightgroupswerefedbyhighfatandhighCholesteroldiet・After4weeks,Modelgroupratsweregivenphysiologicalsalinebyintragastricadministration.Comparisonmedicinetreatmentgroupratsweregivencomparisonmedicinebyintragastrica
8、dministration.MedicineAtreatmentgroupratsweregivenMedicineAbyintragastrieadministration.M
