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1、17.10.06C.MediaandReagentsAOACOfficialMethod995.22(a)Washconcentrate.—Onevialcontains1.0gTris,6.0gNaCl,ListeriainFoods0.1gTween20,and2.0mgthimerosalin25mLH2O.ColorimetricPolyclonalEnzyme(b)Positivecontrol.—OnevialcontainslyophilizedpurifiedLis-ImmunoassayScreeningMethodteriaant
2、igen,0.02ggelatin,0.04gboratebuffer,and0.2mg®(TECRAListeriaVisualImmunoassay[TLVIA])thimerosal.FirstAction1995(c)Controldiluent.—Onevialcontains0.01%saline,0.01gFinalAction1999Tris,1.0mgTween20,and1.0mgthimerosalin6mLHO.2(d)Conjugate.—Twovials.Containslyophilizedanti-Listeria[M
3、ethodisscreeningprocedurefordetectionofListeriaspp.indairyfoods,seafoods,poultry,meats(exceptrawgroundchuck),antibodies,0.1gboratebuffer,0.02ggelatin,and0.1gthimerosal.andleafyvegetables.AssayisnotconfirmatorybecausepolyclonalReconstitutedconjugateisstable30daysat2–8°C.antibodi
4、esmaycross-reactwithasmallpercentageofnon-Listeria(e)Conjugatediluent.—Twovials(13.5mL/vial).Containsorganisms.EnrichmentspositivebyTLVIAmethodmustbe0.2gboratebufferand2.0mgthimerosalinH2O.streakedonselectivemediaandconfirmedbyappropriatebiochemi-(f)Substrate.—Onevialcontainsly
5、ophilized0.01gcalandhemolysistestsasdescribedinBacteriologicalAnalytical2,2¢-azino-di(3-ethylbenzthiazolinesulfonate)and0.1gManual,currentedition,AOACINTERNATIONAL,Gaithersburg,NaH2PO4×2H2O.MD,USA,andinMicrobiologyLaboratoryGuidebook,U.S.De-(g)Substratediluent.—Onevialcontains0
6、.1gaceticacidandpartmentofAgriculture–FoodSafetyInspectionService,Athens,0.003gH2O2inH2O.GA30604,USA.](h)Stopsolution.—Onevialcontains0.15gNaFin6mLH2O.(Caution:Donotpipettestopsolutionbymouth;avoidcontactwithCaution:Pregnantwomenandpersonswhoareimmuno-skinoreyes.Ineventofaspill
7、,sweeporpipettecontentsintoacompromisedbecauseofillness,medication,orad-beakeranddilutewithwater.Diluteremainderofspilledstopsolu-vancedageshouldavoidhandlingthisorganism.tionwithwater.)(i)Testportionadditive.—1.0gTris,0.1gTween,and1.0mgSeeTable995.22fortheresultsoftheinterlabo
8、ratorystudysup-thimerosalinH2O.portingtheacceptanceoft
