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1、AATCCTestMethod103-2004BacterialAlpha-AmylaseEnzymesusedinDesizing,AssayofDevelopedin1962byAATCCCommit-withcare.UsechemicalgogglesorfaceTableI—GuidetoSelectionteeRA41;Jurisdictiontransferredinshield,imperviousglovesandanofSampleWeight1987toCommitteeRA3
2、4,returnedtoimperviousapronduringdispensingandRA41in1993;reaffirmed1965,1970,mixingofiodine;thisisacorrosiveSampleweight1973,1976,1979,1984,2004;editori-chemical.Sampletobeusedallyrevised1985,1986,1991;editori-4.3Aneyewash/safetyshowershouldalpha-amyla
3、semgsampleallyrevisedandreaffirmed1989,1994;belocatednearbyandaself-containedcontentper10mLrevised1999.breathingapparatusshouldbereadily(BAUperg)finaldilutionavailableforemergencyuse.70-2502004.4Exposuretochemicalsusedinthis125-500100proceduremustbecon
4、trolledatorbelow300-90050levelssetbygovernmentalauthorities600-1800251.PurposeandScope[e.g.,OccupationalSafetyandHealth1000-400010Administration’s(OSHA)permissible3000-900051.1Thistestmethodisintendedfortheexposurelimits(PEL)asfoundin296000-18000.02.5a
5、ssayofthebacterialamylasesemployedCFR1910.1000ofJanuary1,1989].In12500-500001commerciallyfortextiledesizing.Itisnotaddition,theAmericanConferenceofapplicabletoproductswhichcontainbeta-GovernmentalIndustrialHygienistsamylaseinadditiontoalpha-amylase.(AC
6、GIH)ThresholdLimitValues(TLVs)comprisedoftimeweightedaverages2.Principle(TLV-TWA),shorttermexposurelimitsKHPO(monobasic)24(TLV-STEL)andceilinglimits(TLV-C)7.4Sodiumphosphate;Na2HPO42.1Dextrogenicamylaseactivityisarerecommendedasageneralguidefor(dibasic
7、)measuredintermsofthedigestiontimeaircontaminantexposurewhichshould7.5Merck’sLintnerStarchrequiredtoproduceacolorchangedenot-bemet(see13.2).ingadefinitestageofdextrinizationofthestarchsubstrate.Theamylasecontent8.Preparations5.UsesandLimitationsofthesa
8、mple,expressedinBacterialAmylaseUnits(BAU),isreadilycalcu-5.1Thecalibrationofthemethodas8.1TestSamplePreparation.latedfromthedextrinizingtime.presentedherefurtherrestrictsitsuseto8.1.1Preparethesamplesolutionbyamylasesofbacterialorigin.
