资源描述：
《Qiagen_RNeasy_Mini_kit说明书》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、FourthEditionApril2006RNeasy®MiniHandbookRNeasyMiniKitForpurificationoftotalRNAfromanimalcells,animaltissues,bacteria,andyeast,andforRNAcleanupRNeasyProtectMiniKitForimmediatestabilizationofRNAinharvestedanimaltissuesandsubsequenttotalRNApurificationRNeasyPlantMiniKitForpurificationof
2、totalRNAfromplantsandfilamentousfungiWWW.QIAGEN.COMTrademarks:QIAGEN®,HotStarTaq®,MinElute®,Omniscript®,Quantiscript®,QuantiTect®,RNAprotect®,RNeasy®,Sensiscript®(QIAGENGroup);ABIPRISM®,AppliedBiosystems®(AppleraCorporationoritssubsidiaries);Agilent®(AgilentTechnologies,Inc.);DU®(Beck
3、manCoulter,Inc.);LightCycler®,TaqMan®(RocheGroup);POLYTRON®(KINEMATICAAG);SYBR®(MolecularProbes,Inc.);Tissue-Tearor™(BioSpecProducts,Inc.);ULTRA-TURRAX®(IKA-AnalysentechnikGmbH).“RNAlater®”isatrademarkofAMBION,Inc.,Austin,TexasandiscoveredbyvariousU.S.andforeignpatents.QIAzolLysisReag
4、entisasubjectofUSPatentNo.5,346,994andforeignequivalents.©2001–2006QIAGEN,allrightsreserved.ContentsKitContents5Storage6QualityControl6ProductUseLimitations6ProductWarrantyandSatisfactionGuarantee7TechnicalAssistance7SafetyInformation8Introduction9Principleandprocedure10Descriptionofp
5、rotocols12EquipmentandReagentstoBeSuppliedbyUser15ImportantNotes18Determiningtheamountofstartingmaterial18Handlingandstoringstartingmaterial20Disruptingandhomogenizingstartingmaterial20EliminatinggenomicDNAcontamination23ProtocolsPurificationofTotalRNAfromAnimalCellsUsingSpinTechnolog
6、y25PurificationofTotalRNAfromAnimalCellsUsingVacuum/SpinTechnology31StabilizationofRNAinHarvestedAnimalTissues36PurificationofTotalRNAfromAnimalTissues39PurificationofTotalRNAfromYeast45PurificationofTotalRNAfromPlantCellsandTissuesandFilamentousFungi52RNACleanup56TroubleshootingGuide
7、58AppendixA:GeneralRemarksonHandlingRNA63AppendixB:Storage,Quantification,andDeterminationofQualityofRNA65AppendixC:FormaldehydeAgaroseGelElectrophoresis67AppendixD:OptionalOn-ColumnDNaseDigestionwiththeRNase-FreeDNaseSet69AppendixE:DNaseDigestionofRNAbeforeRNACleanup71RNeasyMiniHandb
8、ook04
