生物安全第三等级实验室操作程序

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1、生物安全第三等級實驗室人員訓練實驗室操作程序95.3.20版ChainofInfectionPersonalProtectiveEquipmentImmunizationSurveillancePracticesEquipment/FacilityBarriersBarriersPrimarybarrier(主要屏障)：直接隔離人員與對人員有害的生物材料之防護設備Personalprotectiveequipment:口罩、防護衣、手套Laboratoryequipment:安全櫃、排氣櫃SecondaryBarrier(二級屏障）環境設施與工程設計前室、獨立H

2、VAC系統RiskGroupRGIndividualriskCommunityriskProphylaxisortreatment1NoorverylowNoorverylow2ModerateLowUsuallyavailable3HighLowUsuallyavailable4HighHighUsuallyNoneChainofInfectionPersonalProtectiveEquipmentImmunizationSurveillancePracticesEquipment/FacilityRoutesofinfectionMouthaerosols

3、pillhandWound/cutEyePracticesMouthaerosolspillhandEyeWound/cutMouthSkinThroughacut,staborsplashWashimmediatelywithwaterfor5min.Eyesflushwithwateronlyfor10min.Cuts,stabscrubareatobleedRemoveglovesorclothingifrequiredReportaccidentandseekmedicalaidandfollowupSkinPenetratingrubberseptum

4、vialsOpeningpop-toptubesSonicatingtissuesPipettingliquidsCentrifugationVortexingormixingliquidSplash/spillAerosolsEyePipettingPluggedwithcottonMixcarefullyClosetomediumCentrifugationClosedcontainersIntheBSCifneededWellbalancedVortexingCoverspillusingpaperPouronbleachWaitfor10min.Plac

5、espilledmaterialintobiohazardbagCleanupSpillHandPersonalProtectiveEquipmentPersonalProtectiveEquipmentMouthaerosolspillhandEyeWound/cutHandSafetyglassesSafetygogglesFaceshieldsEyeOvergownShoecoversSurgicalscrubsuitsLaboratorycoatPlasticapronsHeadcoverSkinAerosolsrespirator/maskHalffa

6、cerespiratorandcartridgesFullfacerespiratorandsuppliedairBiosafetyCabinetHEPAFilter“Highefficiencyparticulateair”filterTrapsparticulatesonlyEfficiencyof99.97%withparticles0.3umBSCClassIClassIITypeA170%ofairrecirculatedwithinthecabinet30%exhaustedthroughtheHEPAfilterintotheroomTypeA2(

7、B3)SameasA1exceptairisexhaustedoutsidethroughathimbleunitconnection.TypeB130%ofairrecirculated70%exhaustedthroughtheHEPAfiltertotheoutsideviaahardductTypeB2NorecirculationwithtotalexhaustthroughtheHEPAfiltertotheoutsideviaahardductClassIITypeB1ClassIITypeB2ClassIITypeB3ClassIIIFacili

8、ties設施動線：阻隔區