The Switchgrass Genome Tools and Strategies柳枝稷基因组：工具与策略

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1、SPECIALSUBMISSIONSTheSwitchgrassGenome:ToolsandStrategiesMichaelD.Casler,ChristianM.Tobias,ShawnM.Kaeppler,C.RobinBuell,Zeng-YuWang,PeijianCao,JeremySchmutz,andPamelaRonald*AbstractBACKGROUNDSwitchgrass(PanicumvirgatumL.)isaperennialgrassspeciesreceivingsignificantfocusasapotentialbioenergycrop.

2、InthelastSWITCHGRASS(PanicumvirgatumL.)isaperennialC45yrtheswitchgrassresearchcommunityhasproducedageneticgrassnativetoNorthAmericathatisbroadlyadaptedlinkagemap,anexpressedsequencetag(EST)database,asetofforgrowthacrossmuchofNorthandCentralAmerica.singlenucleotidepolymorphism(SNP)markersthatare

3、distributedIthasbeenthefocusofagronomic,breeding,andgenet-acrossthe18linkagegroups,4xsamplingoftheP.virgatumAP13icsresearchsincethe1950s,largelyfocusedondevelop-genomein400-bpreads,andbacterialartificialchromosomementofcultivarsandmanagementsystemsforlivestock(BAC)librariescontainingover200,000c

4、lones.ThesestudiesproductionintheGreatPlainsregionoftheUnitedStateshaverevealedclosecollinearityoftheswitchgrassgenomewith(Vogel,2004).Researchonswitchgrasswasintensifiedinthoseofsorghum[Sorghumbicolor(L.)Moench],rice(Oryza1992whentheU.S.DepartmentofEnergy(USDOE)chosesativaL.),andBrachypodiumdi

5、stachyon(L.)P.Beauv.SwitchgrassresearchershavealsodevelopedseveralmicroarraytechnologiesM.D.Casler,USDA-ARS,U.S.DairyForageResearchCenter,1925forgeneexpressionstudies.SwitchgrassgenomicresourceswillLindenDr.,Madison,WI53706-1108;C.M.Tobias,USDA-ARS,acceleratetheabilityofplantbreederstoenhancepr

6、oductivity,WesternRegionalResearchCenter,Albany,CA94710;S.M.pestresistance,andnutritionalquality.BecauseswitchgrassisaKaeppler,Dep.ofAgronomy,Univ.ofWisconsin,1575LindenDr.,relativenewcomertothegenomicsworld,manysecretsoftheMadison,WI53706;C.R.Buell,Dep.ofPlantBiology,Michiganswitchgrassgenomeh

7、aveyettoberevealed.TocontinuetoStateUniv.,EastLansing,MI48824-1312;Z.Wang,TheSamuelefficientlyexplorebasicandappliedtopicsinswitchgrass,itwillRobertsNobleFoundation,2510SamNoblePkwy.,Ardmore,OKbecriticaltocaptureandexploittheknowle