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1、High-frequencyembryogenesisandregenerationofplantswithhighcontentofgentiopicrosidefromtheChinesemedicinalplantGentianastramineaMaxim.高频胚胎发生以及与龙胆苦昔含量高的中国药用植物麻花苑植株再生。GentianstramineaMaxim.(MahuaQinjiao)isanimportantChinesemedicinalherbthatisrichinsecoiridoids.Toincreasetheproductionofthisp

2、lant,wehavedevelopedanefficienttissueculturesystemforhigh-frequencyplantregeneration.Wehavealsoobtainedplantswithhighsecoiridoidmonomerproductionthroughtissuecultureandsomaticembryogenesis.WehavefoundthattheMBmediumisbetterthantheB5mediumforcallusinduction.Callusesinducedfromimmatureseed

3、saresuperiortothosefromhypocotylsoryoungleavesinregenerationviasomaticembryogenesis.Wehavealsodemonstratedthat2,4-dichlorophenoxyaceticacidisefficientforbothcallusinductionandembryogenesis,indole-3-aceticacidissuitableforembryogeniccallusproliferation,andNAsup6A-(benzyl)-adeninepromotesb

4、othembryodevelopmentandtheaccumulationofgentiopicrosideinthecultures.Regeneratedplantshavebeenselectedforhighgentiopicrosideconten匸Oneplantcontains5.82%ofgentiopicroside,whichishigherthanthecontrolplants(1.20-3.73%).Theregeneratedplantsaregeneticallymorestablethanthecallusesbasedonbothcy

5、tologicalandrandomamplifiedpolymorphicDNAanalyses.高频胚胎发生以及与龙胆苦昔含量高的中国药用植物麻花茏植株再生。秦芜。(MahuaQinjiao)是一种重要的中草药是在secoiridoids丰富。为了提高这家工厂生产，我们开发了一种用于高频高效组织培养植株再生体系。我们亦得到通过组织培养和体细胞胚胎发生与高secoiridoid单体生产厂。我们发现,MB培养基比B5培养基诱导愈伤组织的更好。利用未成熟种子诱导愈伤组织均优于或从下胚轴再牛嫩叶通过体细胞胚胎发牛的。我们还表明，2,4二氯苯氧乙酸是有效率的为愈伤组织诱导和胚

6、胎，口引喙-3乙酸是合适的胚性愈伤组织增殖和"6'燮-(节基)腺卩票吟胚胎发育和促进两在龙胆苦昔的文化积累。再生植株已被选定为龙胆苦廿含量高。一个植物含有龙胆苦廿5.82%,比对照植株(1.20-3.73%)为高。再生植株的基因在两个以上的细胞学和随机扩增多态性DNA分析的基础上老茧稳定。ETHNOPHARMACOLOGYOFMEXICANASTERACEAETraditionalherbalremedieshaveincreasedinpopularityinEuropeandtheUnitedStatesinrecentyearsbuthavealwaysbeeni

7、mportanttopeoplelivinginruralMexicoandtotheirMexicanAmerican/ChicanodescendantsintheUnitedStates・MexicanAmericanpatientswilloftenbeingestingherbalteasatthesametimethattheyarebeingtreatedfortheirailmentswithantibioticsorantiinflammatoryagents.TheplantfamilyAsteraceae(Compo