10-Nucleotides, Oligonucleotides,and Polynucleotides

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1、MolecularBiologyProblemSolver:ALaboratoryGuide.EditedbyAlanS.GersteinCopyright©2001byWiley-Liss,Inc.ISBNs:0-471-37972-7(Paper);0-471-22390-5(Electronic)10Nucleotides,Oligonucleotides,andPolynucleotidesAlanS.GersteinNucleotides...........................................268Nomenc

2、lature:DefactoandDujour..................268WhatMakesaNucleotidePure?.......................269AreSolutionNucleotidesAlwaysMorePureThanLyophilizedNucleotides?............................269AreSolutionNucleotidesMoreStableThanLyophilizedNucleotides?..............................

3、........270DoesYourApplicationRequireExtremelyPureNucleotides?......................................272HowCanYouMonitorNucleotidePurityandDegradation?......................................272TheauthorwouldliketothankAnitaGradowskiofPierceMilwaukeeforcontributingsuchthoroughandh

4、elpfulinformationregardingthepreparationofnucleotidesolutions.SpecialthanksalsotoCicaMinettiandDavidRemetaofRutgersUniversityfordiscussingamethodtocalculatetheextinctioncoefficientofanoligonucleotide.ThecontributionstothischapterbyHowardCoyerandThomasTyre,alsoofPierceMilwaukee,a

5、retoonumeroustolist.267HowShouldYouPrepare,Quantitate,andAdjustthepHofSmallandLargeVolumesofNucleotides?...........273WhatIstheEffectofThermocyclingonNucleotideStability?..........................................275IsThereaDifferencebetweenAbsorbance,A260,andOpticalDensity?....

6、...............................275WhyDoA260UnitValuesforSingle-StrandedDNAandOligonucleotidesVaryintheResearchLiterature?......278Oligonucleotides.......................................279HowPureanOligonucleotideIsRequiredforYourApplication?.....................................

7、..279WhatAretheOptionsforQuantitatingOligonucleotides?..................................279WhatIstheStorageStabilityofOligonucleotides?........280YourVialofOligonucleotideIsEmpty,orIsIt?...........281SyntheticPolynucleotides................................281IsaPolynucleotideId

8、enticaltoanOligonucleotide?.......281HowArePolynucleot