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1、工业卫生与职业病2009年第35卷第1期IndHlth&OccupDis2009,Vol135,No11·1·【论著】枸杞多糖对大鼠心理应激的作用1211111周健,张银娥,杨惠芳,朱玲勤,李宏辉,高晓燕,张鹏举(11宁夏医学院公共卫生学院,宁夏银川750004;21宁夏疾病预防控制中心,宁夏银川750004)【摘要】目的探讨枸杞多糖对大鼠慢性心理应激行为、神经内分泌和细胞因子的影响。方法健康成年SD大鼠60只,雌雄各半,随机分为6组,通过旷场实验和悬尾实验观察枸杞多糖调节后各组大鼠的行为变化;采用放射免疫法测定
2、血清皮质醇、IL21、IL22和TNF2α的含量。结果与应激组比较,枸杞多糖低剂量+应激组和枸杞多糖高剂量+应激组大鼠血清皮质醇含量明显降低(P<0105);血清IL21、IL22和TNF2α的含量明显升高(P<0105);旷场实验及悬尾实验结果显示,与应激组比较,枸杞多糖低剂量+应激组和枸杞多糖高剂量+应激组大鼠潜伏期缩短,穿格数和修饰时间增加(P<0105),静止时间明显减少(P<0105),挣扎次数明显增多(P<0105)。结论枸杞多糖能够调节下丘脑-垂体-肾上腺皮质(HPA)轴的适应性,降低皮质醇的过量释
3、放,维持免疫功能稳定,调节心理应激水平。【关键词】枸杞多糖;慢性心理应激;皮质醇;IL21;IL22;TNF2α[中图分类号]R13[文献标识码]A[文章编号]100027164(2009)0120001203LyciumBarbarumPolysaccharidesinRatsunderChronicPsychologicalStress333ZHOUJian,ZHANGYin2e,YANGHui2fang,ZHULing2qin,333LIHong2hui,GAOXiao2yan,ZHANGPeng2ju(3
4、SchoolofPublicHealth,NingxiaMedicalCollege,Yinchuan750004China)【Abstract】ObjectiveTostudytheeffectoflyciumbarbarumpolysaccharides(LBP)onbehavior,neuroendocrineandimmunefactorinratsunderchronicpsychologicalstress.MethodsSixtySDratsweredividedintosixgroupsandgi
5、venlyciumbarbarumpolysaccharides.Theirethologicalchangesandthecontentsofcortisol,IL21,IL22,TNF2αinserumofratswerealsodetected.ResultsTheserumcortisollevelsofLBPlowdose+stressgroupandLBPhighdose+stressgroupweresignificantlylowerthanthoseofthestressgroup(P<0105
6、),whiletheserumIL21,IL22andTNF2αweresignificantlyhigherthanthoseofthestressgroup(P<0105).Comparedwiththestressgroup,theopen2fieldtestandthetail2suspensiontestshowedthattheincubationperiodwasshortened(P<0105),thestilltimewassignificantlydecreased(P<0105),andth
7、efrequencyofstrugglingwasincreased(P<0105).ConclusionsTheLBPcouldimprovetheadaptationofhypothalamicpituitaryadrenocorticalaxis,reducetheexcessivereleaseofcortisol,keepthestabilityofimmunefunctionandregulatethelevelofpsychologicalstress.【Keywords】Lyciumbarbaru
8、mpolysaccharides;Chronicpsychologicalstress;Cortisol;Interleukin21;Interleukin22;TNF2α过度及慢性的心理应激均可导致机体疲乏、焦慢性心理应激大鼠的行为、神经内分泌及免疫功能虑、压抑、工作能力下降和机体免疫力下降。枸杞的影响。多糖是从枸杞中提取的一种生物活性物质,具有促进T、B、NK等免疫细
