Connection between biomechanics and cytoskeleton structure of lymphocyte and

《Connection between biomechanics and cytoskeleton structure of lymphocyte and》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、Micron41(2010)257–262ContentslistsavailableatScienceDirectMicronjournalhomepage:www.elsevier.com/locate/micronConnectionbetweenbiomechanicsandcytoskeletonstructureoflymphocyteandJurkatcells:AnAFMstudyXiaofangCai,XiaoboXing,JiyeCai*,QianChen,ShixianWu,FeichengHu

2、angChemistryDepartment,JinanUniversity,Guangzhou510632,PRChinaARTICLEINFOABSTRACTArticlehistory:Themechanicalpropertiesofcellsareimportantformanycellularprocesses.Here,atomicforceReceived30June2009microscopy(AFM)andlaserscanningconfocalmicroscopy(LSCM)werecarri

3、edouttocharacterizeReceivedinrevisedform6August2009lymphocyteandJurkatcells.Theaverageelasticmodulusoflymphocyteis1.240.09kPa,whichisAccepted7August2009almosttwofoldhigherthanthatofJurkatcell(0.510.06kPa).LSCMimagesofsub-membranecytoskeletonshowedasignificantd

4、ifferenceintheorganizationoftheirF-actinstructures.LymphocytecellshadmoreandKeywords:thickeractinbundlesthanthatofJurkatcells.LymphocyteandJurkatcellsafteraddingtheF-actinAtomicforcemicroscopy(AFM)destabilizingagentCytochalasin-B(Cyt-B)werealsoinvestigatedbyAFM

5、.AdecreaseintheelasticmodulusLymphocytecelloflymphocytefromavalueof1.240.09kPadownto0.340.04kPafor24hwasobserved,andthatofJurkatcellJurkatcelldecreasedfrom0.510.06kPato0.230.04kPa.WereallybelievethatthistechnologywillbeYoung’smodulusLaserscanningconfocalmic

6、roscopy(LSCM)usedforcancerdetectionandopensadoortostudythebiophysicalpropertiesofsignalingdomainsCytochalasin-B(Cyt-B)extendingfromthecellsurfacetodeeperpartsofthecell.ß2009ElsevierLtd.Allrightsreserved.1.IntroductionLim,2007).Thecellularmechanicalpropertiesrefl

7、ecttheirdiseasedstates,andAFMisapowerfultooltodetecttheseLymphocytecellisoneofthekeyplayersintheadaptivechanges(Costa,2003–2004;Ingber,2003;Lim,2006;Sureshetal.,immunesystem.Itservesavarietyoffunctionsincludingthe2005;Huangetal.,2004;WangandThampatty,2003).Rese

8、archrecognitionofspecificforeignantigensinthebody,andtheintobiomechanicsatthecellularormolecularlevelsofsomeactivationanddeactivationofotherimmunecellsordirectlyhumandiseases