2008 Mol Cloney Tech

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1、MolecularColonyTechnique:ANewToolforBiomedicalResearchandClinicalPracticeAlexanderB.ChetverinandHelenaV.ChetverinaInstituteofProteinResearchoftheRussianAcademyofSciences,Pushchino,MoscowRegion,Russia142290I.Introduction..................................

2、.............................................220II.DetectionofAirborneRNAs..........................................................221A.AMysteriousEnzyme..............................................................221B.ThePuzzleofSpontaneousRNASynthes

3、is...................................222C.InventionoftheMolecularColonyTechnique...............................223III.MonitoringReactionsBetweenSingleMolecules................................225A.Cell‐FreeSystemforRNARecombination.......................

4、.............225B.SpontaneousRearrangementsinRNASequences...........................226C.PossibleRoleofSpontaneousRNARecombination.........................227IV.Cell‐FreeGeneCloning................................................................228A.Gen

5、eCloningandExpressioninMolecularColonies.......................228B.AdvantagesoftheMCTCloning.................................................230C.ComparisontoOtherInVitroCloningSystems..............................231V.MolecularColoniesasaPrecellularFo

6、rmofLife................................233VI.MolecularColonyDiagnostics........................................................235A.MCTAssayofDNAandRNATargets..........................................235B.VisualizingMolecularColonies...................

7、................................236C.DetectionofDNAandRNATargetsinClinicalSamples..................238D.QuantitativeInSituAssay.........................................................239VII.GeneandGeneExpressionAnalysis.................................

8、................241A.SequencingonMolecularColonies.............................................241B.Single‐NucleotidePolymorphism................................................242C.AlternativeSplicing............................