资源描述:
《Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre‐ribosomal RNA processing》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ResearchMaizeUrb2proteinisrequiredforkerneldevelopmentandvegetativegrowthbyaffectingpre-ribosomalRNAprocessing1,2,3221,2113HongqiuWang,KaiWang,QingguoDu,YafeiWang,ZhiyuanFu,ZhanyongGuo,DingmingKang,21Wen-XueLiandJihuaTang1NationalKeyLaboratoryofWheatandMaizeCropScienc
2、e,CollaborativeInnovationCenterofHenanGrainCrops,CollegeofAgronomy,HenanAgriculturalUniversity,Zhengzhou23450002,China;NationalEngineeringLaboratoryforCropMolecularBreeding,InstituteofCropScience,ChineseAcademyofAgriculturalSciences,Beijing100081,China;BeijingKeyLabor
3、atoryofCropGeneticImprovement,CollegeofAgricultureandBiotechnology,ChinaAgriculturalUniversity,Beijing100193,ChinaSummaryAuthorsforcorrespondence:Ribosomebiogenesisisafundamentalprocessineukaryoticcells.AlthoughUrb2proteinJihuaTanghasbeenimplicatedinribosomebiogenesi
4、sinyeast,theUrb2domainislooselyconservedTel:+8637163558377betweenplantsandyeast,andthefunctionofUrb2proteininplantsremainsunknown.Email:tangjihua1@163.comHere,weisolatedamaizemutant,designatedasurb2,withdefectsinkerneldevelopmentWen-XueLiandvegetativegrowth.Positiona
5、lcloningandtransgenicanalysisrevealedthaturb2encodesTel:+861082105799anUrb2domain-containingprotein.Email:liwenxue@caas.cnComparedwiththewild-type(WT),theurb2mutantshoweddecreasedratiosof60S/40SReceived:2September2017and80S/40Sandincreasedratiosofpolyribosomes.Thepre
6、-rRNAintermediatesof35/33SAccepted:18January2018rRNA,P-A3and18S-A3weresignificantlyaccumulatedintheurb2mutant.Transcriptomeprofilingoftheurb2mutantindicatedthatZmUrb2affectstheexpressionofanumberofribosome-relatedgenes.WefurtherdemonstratedthatnaturalvariationsinZmUrb2a
7、resig-NewPhytologist(2018)doi:10.1111/nph.15057nificantlyassociatedwithmaizekernellength.Theoverallresultsindicatethat,byaffectingpre-rRNAprocessing,theUrb2proteinisrequiredforribosomebiogenesisinmaize.Keywords:kerneldevelopment,maize(Zeamays),pre-rRNA,ribosome,Urb2.e
8、tal.,2015b),indicatingthatcertainspecificcharacteristicsofIntroductionribosomebiogenesisexistinplants.Ribosomesynthesisisaner