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1、AngewandteChemieInternationalEdition:DOI:10.1002/anie.201508288HybridMaterialsHotPaperGermanEdition:DOI:10.1002/ange.201508288BreakableHybridOrganosilicaNanocapsulesforProteinDeliveryEkoAdiPrasetyanto,*AlessandroBertucci,DedySeptiadi,RobertoCorradini,PabloCastro-Hartmann,andLuisaDeCola*Abstract:The
2、directdeliveryofspecificproteinstolivecellsfunctionalnature,reducingtheneedforrepetitiveinjections,promisesatremendousimpactforbiologicalandmedicalandwithoutcompromisingthebiologicalactivityofthe[4]applications,fromtherapeuticstogeneticengineering.How-protein.ever,theprocessmostlyinvolvestedioustec
3、hniquesandoftenProteinstabilizationfordeliverypurposeshasmainlyrequiresextensivealterationoftheproteinitself.Hereinwebeenachievedbymicro-andnano-encapsulation(e.g.,within[5]reportastraightforwardapproachtoencapsulatenativeliposomes,polymeric,orinorganicstructures)andbiocon-proteinsbyusingbreakableo
4、rganosilicamatricesthatdisinte-jugation(i.e.,covalentlylinkingproteinswithwater-solublegrateuponexposuretoachemicalstimulus.Thebiomolecule-polymersorsimplycrosslinkingproteinstoformstable[1a,6]containingcapsulesweretestedfortheintracellulardeliveryofparticlecomplexes).Invitroproteindeliveryhasbeenh
5、ighlycytotoxicproteinsintoC6gliomacells.Wedemonstrateaddressedbyusingprotein-coatedgold-nanoparticle-stabi-thattheshellisbroken,thereleaseoftheactiveproteinsoccurs,lizednanocapsules,howevertheproteinswerestillexposedto[7]andthereforeourhybridarchitectureisapromisingstrategytothesurroundingenvironme
6、nt.Encapsulationstrategiesdeliverfragilebiomacromoleculesintolivingorganisms.havereceivedmoreandmoreattentionandsomeexamples[8]ofdiversesofthoststructureshavebeenreported.MostlyIntracellulardeliveryofparticularproteinsholdsanimpor-polymericassemblieshavingbiodegradableor,evenbetter,tantroleinbiolog
7、yandmedicine,fromrestoringthefunctionstimuli-responsivenetworksforcontrolledreleasehavebeen[9]ofinterest,producinghighlyspecificmoleculesinsitu,todescribed.Howeverthisencapsulationstrategymaysufferregulatin
