欢迎来到天天文库
浏览记录
ID:40204944
大小:24.76 KB
页数:4页
时间:2019-07-25
《基质金属蛋白酶1ELISA试剂盒,兔ELISA试剂盒说明书》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、基质金属蛋白酶1ELISA试剂盒,兔ELISA试剂盒说明书基质金属蛋白酶1ELISA试剂盒,兔ELISA试剂盒说明书供应商:上海乔羽生物有限公司基质金属蛋白酶1ELISA试剂盒,兔ELISA试剂盒说明书Kitperformance1sensitivity:theminimumconcentrationislessthan1standard.Linearityofdilution.SamplelinearregressionandtheexpectedconcentrationcorrelationcoefficientRvalu
2、eis0.990.2specificity:noreactionwithothercytokines.3repeatability:thevariationcoefficientoftheplateandtheplatearealllessthan10%.Resultjudgmentandanalysis1,theinstrumentvalue:YuBo450nmELISAodreadtheholeontheinstrument2,totheODvalueastheordinate,y,correspondingHLA-B27s
3、tandardconcentrationastheabscissa,x,dothecorrespondingcurve,HLA-B27contentinthesamplecanbeaccordingtoitsODvaluebystandardcurveconversionoutcorrespondingconcentration.3,valuerange:0-8.0IU/ml4,sensitivity:0.01IU/ml基质金属蛋白酶1ELISA试剂盒,兔ELISA试剂盒说明书Kitcomposition:Sealingfilm
4、:2(48)/2(96)Specifications:1copiesSealingbag:1Standard:2700ng/L0.5*0.5ml*1bottles,1bottlesarestoredin2-8ELISApackageisboard:1X48x9612-8storedinSampledilution:3ml*1ml*1bottlesof6bottlesarestoredin2-8Coloragent:Liquid3ml*1ml*6bottlesof1bottlesin2-8ColorreagentBLiquid3m
5、l*1ml*1bottle6bottlesstoredin2-8Terminationsolution:3ml*6ml*1bottlesand1bottlesarestoredin2-8Concentratedlaundryliquid:(2*20)*1bottles(20ml*30)*1bottlesstoredin2-8elisa试剂盒价格,elisa试剂盒说明书,elisa检测试剂盒基质金属蛋白酶1ELISA试剂盒,兔ELISA试剂盒说明书ELISA技术原理:以免疫学反应为基础,将抗原、抗体的特异性反应与酶对底物的高效催化
6、作用相结合起来.1.抗原或抗体的固相化及抗原或抗体的酶标记。2.结合在固相载体表面的抗原或抗体仍保持其免疫学活性。3.酶标记的抗原或抗体既保留其免疫学活性,又保留酶的活性。4.受检标本与固相载体表面的抗原或抗体起反应。再加入酶标记的抗原或抗体,也通过反应而结合在固相载体上。5.此时固相上的酶量与标本中受检物质的量呈一定的比例。6.加入酶反应的底物后,底物被酶催化成为有色产物,产物的量与标本中受检物质的量直接相关,故可根据呈色的深浅进行定性或定量分析。测定方法具有很高的敏感度(pg-ng/ml水平),并且重复性好。基质金属蛋白酶1
7、ELISA试剂盒,兔ELISA试剂盒说明书Operationalmattersneedingattention:Thereagentshouldlabelstorageinstructions,returntoroomtemperaturebeforeuse.Afterthinningofthestandardproductsshouldbediscarded,cannotbesaved.No-intheexperimentoftheplateshouldbeimmediatelyputbackthebag,sealedtopr
8、eventspoilage.-nootherreagentsshouldbepackagedorcovered.Donotmixdifferentbatchesofreagents.Beforeuse.]usingdisposablesuctionheadtoa
此文档下载收益归作者所有